The surface-enhanced Raman spectroscopy (SERS) substrate enhances the Raman scattering light from molecules, making high-sensitive Raman spectroscopic analysis possible. A chip of metallic nano/sub-micro structure is mounted on Hamamatsu’s original handling plate to protect its active area. The active area of the handling plate is of a well structure for easily attaching solution or the like. It is noted this is a disposable product and cannot be reused.