A12801-01W-VIEW GEMINI Image splitting optics

a12801-01 product photo

Catalog [1 MB/PDF]

Technical note [1 MB/PDF]

The W-VIEW GEMINI is an image splitting optics which provides one pair of dual wavelength images separated by a dichroic mirror onto a single camera. Simultaneous image acquisition of dual wavelength images allows you high speed ratio metric imaging and other multiple fluorescence applications.

Features

Signal from your sample can be hard to get don't waste it.

The W-VIEW GEMINI provides excellent transmittance across a broad range of wavelengths. Exceptional performance from 400 nm to 800 nm is enabled by our proprietary lens system. When used with "imaging grade" filters, the system is optimized for low light imaging.

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Use all the pixels you've purchased

The W-VIEW GEMINI is designed to take advantage of the wide field of view provided by sCMOS cameras. Up to 13 mm × 6.4 mm F.O.V. and resolution of up to approximately 2000 pixels × 1000 pixels for each image.


Choose your own filters

Because it is compatible with commercially available filters, the W-VIEW GEMINI allows for great wavelength flexibility. Choose the dichroic, bandpass and neutral density filters that work for your experimental question.


Always ready, but never in the way.

Our "Bypass mode" (patent pending) means that by simply removing the dichroic mirrors, the camera sees the image as though it was connected directly to the microscope. You can switch between standard imaging and dual wavelength imaging without having to reconfigure the camera or remove the W-VIEW GEMINI.


Chromatic aberration correction mechanism

The W-VIEW GEMINI has a correction lens unit in the long wavelength path and it can improve the magnification difference of two wavelength images caused by chromatic aberration.
The right images show an example of the magnification difference caused by chromatic aberration is improved by the correction lens unit.

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Symmetrically balanced design

Designed for stability on both inverted and upright microscopes, the W-VIEW GEMINI's C-mount connectors are placed along a linear axis. And with its compact form factor, it integrates smoothly on a wide range of research microscopes.


Fast and straight forward alignment

The W-VIEW GEMINI is designed to be easily adjusted when used with any camera. And, when using a Hamamatsu camera, the included "W-VIEW Adjustment" software makes the process even faster by simultaneously displaying and magnifying nine strategic points on the concentric chart (also included). This visual feedback lets the user dial in alignment quickly and accurately.

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Light path

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Applications

Wide field of view and high speed Ca2+ imaging of YC3.60 expressing ASER neuron in C. elegans

The combination of W-VIEW GEMINI and ORCA-Flash4.0 series sCMOS camera realizes wide field of view dual wavelength imaging. The combination enables FRET measurement of entire single neuron (cell body and dendrite) of C.elegans even under measuring conditions with high NA and magnification objective lens.

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Fast Ca2+ imaging of YC3.60 expressing HeLa cell

Ca2+ dynamics of HeLa cell by histamine stimulation was observed at 33.3 frames/s by the combination of W-VIEW GEMINI and ImagEM X2 EM-CCD camera.

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Sample images

3D multi-color observation of Amoeboid movement and the cytoplasm flow

 

Simultaneous two color imaging of the beating zebrafish heart

 

Ca2+ Imaging of the nematode C. elegans

 

Fast Ca2+ imaging of YC3.60 expressing HeLa cell

 

Simultaneous intracellular Ca2+ and phase contrast imaging of spontaneously beating hiPS-cardiomyocytes.

 

Ratio imaging of hiPS-cardiomyocytes membrane potential with di-4-ANEPPS.

 

An analysis of the spatio-temporal activity in a salt-sensing neuron of the nematode C. elegans

 

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Publications

Authors Title Source
Tadasu Nozaki, Ryosuke Imai, Mai Tanbo, Ryosuke Nagashima, Sachiko Tamura, Tomomi Tani, Yasumasa Joti, Masaru Tomita, Kayo Hibino, Masato T. Kanemaki, Kerstin S. Wendt, Yasushi Okada, Takeharu Nagai, Kazuhiro Maeshima Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging DOI: http://dx.doi.org/10.1016/j.molcel.
2017.06.018
Shigenori Inagaki, Hidekazu Tsutsui, Kazushi Suzuki, Masakazu Agetsuma, Yoshiyuki Arai, Yuka Jinno, Guirong Bai, Matthew J. Daniels, Yasushi Okamura, Tomoki Matsuda & Takeharu Nagai Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging Scientific Reports 7,
Article number: 42398 (2017)
doi:10.1038/srep42398
Masahiro Nakano, Yoshiyuki Arai, Ippei Kotera, Kohki Okabe, Yasuhiro Kamei, Takeharu Nagai Genetically encoded ratiometric fluorescent thermometer with wide range and rapid response PLoS One
Adam L. Nekimken, Holger Fehlauer, Anna A. Kim, Sandra N. Manosalvas-Kjono, Purim Ladpli, Farah Memon, Divya Gopisetty, Veronica Sanchez, Miriam B. Goodman, Beth L. Pruitt and Michael Krieg Pneumatic stimulation of C. elegans mechanoreceptor neurons in a microfluidic trap Lab on a Chip
DOI:10.1039/C6LC01165A
Jie Yang, Derrick Cumberbatch, Samuel Centanni, Shu-qun Shi, Danny Winder, Donna Webb & Carl Hirschie Johnson Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing Nature Communications 7,
Article number: 13268 (2016)
doi:10.1038/ncomms13268
Tomoki Yoshida, Akira Kakizuka and Hiromi Imamura BTeam, a Novel BRET-based Biosensor for the Accurate Quantification of ATP Concentration within Living Cells SCIENTIFIC REPORTS
doi: 10.1038/srep39618
Marina Theodosiou, Moritz Widmaier, Ralph T Böttcher, Emanuel Rognoni, Maik Veelders, Mitasha Bharadwaj, Armin Lambacher, Katharina Austen, Daniel J Müller, Roy Zent, Reinhard Fässler Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin DOI: http://dx.doi.org/10.7554
/eLife.10130
Published January 28, 2016
P Jönsson, B Jönsson Hydrodynamic Forces on Macromolecules Protruding from Lipid Bilayers Due to External Liquid Flows Langmuir, 2015, 31 (46), pp 12708–12718 DOI: 10.1021/acs.langmuir.5b03421 Publication Date (Web): November 2, 2015
Michaela Mickoleit, Benjamin Schmid, Michael Weber, Florian O Fahrbach, Sonja Hombach, Sven Reischauer, Jan Huisken High-resolution reconstruction of the beating zebrafish heart NATURE METHODS 11, 919–922 (2014) doi:10.1038/nmeth.3037
Juette MF, Terry DS1, Wasserman MR, Zhou Z, Altman RB, Zheng Q, Blanchard SC. The bright future of single-molecule fluorescence imaging CURRENT OPINION IN CHEMICAL BIOLOGY. 2014 Jun 20;20C:103-111. doi: 10.1016/j.cbpa.2014.05.010.

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Video tutorials

The following videos are intended to give an overview of the features of the W-VIEW GEMINI. An introduction is followed by sections on the light path, Bypass mode and finally instruction on how to pack the optic for shipping should you need to move it.

 

01 Introduction

 

02 How it Works and Strategic Advantages

 

03 Bypass Mode Explained

 

04 Shipping Dos & Don’ts

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Configuration

a12801-01 system configuration

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Specifications

Product number  A12801-01 
Structure  1 camera type, C-mount to C-mount linear structure, For Uplight / Inverted microscope 
Input mount  C-mount (female) 
Output mount  C-mount (male) 
Relay lens magnification  1.0 
Field of view*1  13 mm × 6.4 mm (W-VIEW mode)
13 mm × 13 mm (Bypass mode) 
Mode  W-VIEW mode / Bypass mode*2 
Transmittance wavelength range*3  400 nm to 800 nm 
Transmittance (Typ.)*4  97 % 
Dichroic mirror*5 *6  Compatible with 25.2 × 35.6 
Bandpass filter*5 *6  Compatible with φ25.4 fillter 
ND filter*5 *6  Compatible with φ25.4 fillter 
Chromatic aberration correction mechanism  Correction lens unit*7*8*9 
Application  For fluorescence imaging with microscope 
Ambient operating temperature  0 ℃ to +40 ℃ 
Ambient operating humidity  70 % max. (With no condensation) 
Ambient storage temperature  -10 ℃ to +50 ℃ 
Ambient storage humidity  70 %max. (With no condensation) 
*1:Vignetting may occur when used with a relay lens or variable magnification lens. Please check with your Hamamatsu representative to confirm this point before purchase.
*2:Mode in which dichroic mirror, etc. are removed from the light path and the image from the microscope is projected to the camera without alteration.
*3:All are values in the bypass mode.
*4:Value at peak wavelength in the bypass mode.
*5:Because dichroic mirror, band-pass filter and ND filter are not included with the W-VIEW GEMINI, they must be purchased separately. Use an "imaging grade" dichroic mirror and bandpass fillters. See "Dichroic mirror and Bandpass filter specifications". A set which includes one Dichroic holder (empty) and one Filter holder (empty) is included with the W-VIEW GEMINI. Additional sets can be purchased by ordering part number A12802-01.
*6:For the usable size, see “Size of dichroic mirror and filters”.
*7:This is to improve the magnification difference caused by a chromatic aberration. The position difference of two wavelength images caused by the distortion aberration is not improved.
*8:Since the FOCUS knob is designed to improve the axial chromatic aberration caused by this optics, its effect is very limited.
*9:Dual focal plane imaging is not possible.

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Dichroic mirror and Bandpass filter specifications

To utilize the performance of the W-VIEW GEMINI's finely corrected optical system, we recommend using only dichroic mirror and bandpass filters of the highest quality. Appropriate dichroic mirror and bandpass filters can be purchased from companies like Semrock. Be sure to ask for their "imaging grade" filters.

Recommended examples of Semrock filters

for W-VIEW GEMINI

CFP/YFP FRET Imaging
Bandpass Emitter 1 FF01-483/32-25
Bandpass Emitter 2 FF01-542/27-25
Dichroic mirror FF509-FDi01-25×36
GFP/DsRED Dual Band Imaging
Bandpass Emitter 1 FF01-512/25-25
Bandpass Emitter 2 FF01-630/92-25
Dichroic mirror FF560-FDi01-25×36
Cy3/Cy5 FRET Imaging
Bandpass Emitter 1 FF01-593/40-25
Bandpass Emitter 2 FF01-676/29-25
Dichroic mirror FF640-FDi01-25×36

for Microscope

CFP/YFP FRET Imaging
Excitation FF02-438/24-25
Dichroic mirror FF458-Di02-25×36
Emission BLP01-R458-25
GFP/DsRED Dual Band Imaging
Excitation FF01-468/553-25
Dichroic mirror FF493/574-Di01-25×36
Emission FF01-512/630-25
Cy3/Cy5 FRET Imaging
Excitation FF01-531/40-25
Dichroic mirror FF562-Di03-25×36
Emission BLP02-R561-25
 
 

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Size of dichroic mirror and filters

  Size (mm) / Tolerances (mm) Thickness (mm)
Dichroic mirror 25.1×35.5 to 26.1×38.1 0.9 to 2.1
Bandpass filter φ25.4 +0/-0.6 Shortwavelength 5.0(Max.)*
Longwavelength 6.0(Max.)*
ND filter

* The value is total thickness of a bandpass filter and ND filter.

 

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Dimensions

a12801-01 dimensional outline

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Related information

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Disclaimer
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