A12801-10W-VIEW GEMINI-2C Image splitting optics
Dual camera image splitting optics done right
Dual channel optics are simple… in principle. In practice, designing a device that enables quick, stable alignment and delivers images with minimal distortions over a large field of view requires attention to detail. The W-VIEW GEMINI-2C gets the details right and more. Building on our imaging and optical experience, the W-VIEW GEMINI-2C is the first dual camera, dual channel system engineered with super resolution quality, while simultaneously offering versatility, expandability and ease of use. The W-VIEW GEMINI-2C turns a simple optical concept into an elegant and useful tool that can help maximize the information content of every image acquisition.
Unrivaled Optical Quality Provides Superior Images
Custom Designed Lenses Optimized for Fluorescent Imaging
Using custom designed optics, our engineers fully optimized system performance, offering super resolution quality by minimizing point spread function (PSF) degradation, field curvature and wave front aberration.
Wide Field of View (20 mm for standard imaging, 12 mm for diffraction-limited imaging)
Maintaining optical quality at the edges of the field demands extra care. The W-VIEW GEMINI-2C delivers excellent performance over the entire field of two ORCA-Flash4.0 sCMOS cameras and diffraction-limited performance within the center 12 mm diameter FOV.
Ultra-Low Distortion (0.05%), High Spatial Uniformity (98%), High Transmission (98 % @ 450 nm to 800nm)
This unmatched level of optical performance delivers bright, even, chromatic aberration-corrected images to both cameras.
Robust Opto-Mechanical Design for Ease of Alignment and Versatility
Dual Channel x, y, z and Rotational Alignment
The W-VIEW GEMINI-2C makes alignment easy and stable. Included alignment software provides direct visual feedback when using Hamamatsu cameras. An optional grid chart fits directly into the primary imaging plane making it possible to align images in the absence of feedback at the sample position.
Standard Filters and (Dichroic) Beamsplitters
All filter and beamsplitter holders are designed to industry standard specifications, allowing researchers to freely choose their optical components.
Bypass and Camera Switching Mode
It’s easy to bypass all the optics of the W-VIEW GEMINI-2C, simply remove the filters and beamsplitter and insert the port covers. In addition, by using a 100 % mirror instead of a beamsplitter, the W-VIEW GEMINI-2C can be used to switch between two different cameras.
With input and output C-mounts, the W-VIEW GEMINI-2C is compatible with and easily attached to standard inverted microscopes.
Attention to Detail Enables Advanced Imaging Applications
Simulations of dual wavelength, dual camera imaging optics that use two commercially available doublet lenses, expose issues with astigmatism and field curvature, making such devices unacceptable for super resolution applications. To overcome this optical limitation, the W-VIEW GEMINI-2C uses custom designed and manufactured lenses that minimize wavefront aberrations (to less than λ/14 rms) to maintain the diffraction limit.
Lightsheet, Single Molecule Imaging and Super Resolution Microscopy
The W-VIEW GEMINI-2C was specifically developed to bring the temporal advantage of simultaneous dual channel imaging to cutting edge applications. To faithfully reproduce sample position, intensity and distribution characteristics across two sCMOS cameras requires refined optics. Using simulations of a spot projected through the optical system, the resolution of the W-VIEW GEMINI-2C, as shown by the size and shape of the beam at 0 mm, 3 mm and 6 mm from optical center, is significantly better. This data is shown for both high magnification (Fig 2), typical for single molecule and super resolution experiments, and low magnification (Fig 3), typical for lightsheet imaging systems.
Tri-Color and Quad-Color Imaging
The 4f configuration of the optics makes it possible to connect multiple W-VIEW GEMINI-2Cs for full-field, tri-color, threecamera imaging. Alternatively, the addition of two single camera W-VIEW GEMINI devices provides quad channels imaged onto two ORCA-Flash4.0 series cameras.
Modular Design Supports Expansion into Engineered Point Spread Function Imaging
The W-VIEW GEMINI-2C is the first dual channel imaging device supporting engineered point spread function (PSF) imaging. For example, by using phase masks, (offered by Double Helix, www.doublehelixoptics.com) to create known modifications to the PSF, additional z position information can be extracted from a single plane permitting rapid 3D imaging and 3D tracking. Other mask designs can provide different functionalities. With the W-VIEW GEMINI-2C’s triaxial adjustment mount and field lens unit installed, the phase masks are positioned into the relayed pupil plane. The field lens determines the macro positioning of the phase mask for each microscope system and triaxial adjustment mount provides fine x, y, z adjustment for each wavelength. With the complete W-VIEW GEMINI-2C PSF module installed, plus Double Helix phase masks and image processing software, any inverted microscope evolves into an advanced two color, 3D imaging system with super resolution capabilities.
Enabling engineered PSF Imaging with the W-VIEW GEMINI-2C
Engineered PSF imaging requires the phase masks be in the pupil plane. This is problematic since the microscope pupil plane is in the objective lens. To make engineered PSF imaging practical, it’s necessary to relay the pupil plane to an accessible location. However, each microscope system has different optics, requiring flexibility in the positioning of the projected pupil plane. Hamamatsu has overcome this problem with a patent pending design that inserts a standard field lens near the primary image plane, bringing the actual pupil plane in line with the ideal pupil plane for most microscopes and objective lenses. By choosing the appropriate focal length field lens, the phase masks (set inside the triaxial adjustment mount) align into the pupil plane. To easily visualize each phase mask, a Bertrand lens projects the pupil plane/phase mask onto the camera. All the necessary components for engineered PSF imaging are listed on inside back cover. Although not included with the W-VIEW GEMINI-2C, Hamamatsu can recommend commercially available phase masks for your application and field lenses for your microscope brand and configuration.
|Structure||Dual camera, parallel arrangement for inverted microscopes|
|Input mount||C-mount, female|
|Output mount||C-mount, male|
|Relay lens magnification||1.0x|
|Relay lens focal length||130 mm|
|Field of view*6||φ20 mm
φ12 mm (Diffraction limitted)*3
|Field of view, diffraction limited*7||φ12 mm|
|Transmittance wavelength range*1||450 nm to 800 nm|
|Maximum transmittance (Typ.)*2||98 %|
|Dichroic mirror size||25.1 x 35.5 to 26.1 x 38.1|
|Dichroic mirror thickness*8||0.9 mm to 3.1 mm|
|Band-pass and ND filter size||φ25.4|
|Band-pass and ND filter tolerance||+0 mm, -0.6 mm|
|Max thickness of band pass and ND filters*9||6 mm|
|Longitudinal chromatic aberration correcting mechanism||Yes|
|Lateral chromatic aberration correcting mechanism||Optional|
|Mirror image inversion||No|
|X-Y position adjustment||Yes|
|Distortion (typ.)*4||0.05 %|
|Lateral color shift (typ.)*4,5||6 μm|
|Uniformity (typ.)*3||98 %|
|Ambient operating temperature||0 ℃ to +40 ℃|
|Ambient storage temperature||-10 ℃ to +50 ℃|
|Ambient operating and storage humidity||70 %max. (With no condensation)|
*2: Peak wavelength in bypass mode
*3: Min/max difference of images obtained with and without W-VIEW GEMINI-2C optics ORCA-Flash4.0 V3 camera used for imaging
*4: Values calculated without objective lens
*5: Amount of peripheral position shift when aligned at center
*6: Vignetting may occur if used with relay or variable magnification lens
*7: Simulation value for 0.025 imaging side N.A. of objective lens
*8: Recommend 3 mm thick, high flatness on reflection side; typically called “imaging grade”
*9: Sum of bandpass and ND filters used