C13299FDSS/µCELL Functional Drug Screening System

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Catalog [1 MB/PDF]

FDSS/μCELL is a laboratory screening system that compactly integrates technologies developed in drug discovery screening, enabling a purpose-built system that is simple to use.
Simultaneous measurement and analysis of the kinetics of a sample’s fluorescence or luminescence intensity in all wells at the time of compound addition are made possible by the high sensitivity two-dimensional sensor (camera) and dispenser head (96 tip type/384 tip type). Screening various compounds at high throughput is enabled by measurement under the same conditions with no time lag between wells.

 

FDSS Publication List

Features

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Applications

1. GPCR

GPCRs (G protein-coupled receptors) play a major role in cell signaling, and many GPCR-targeted medical drugs have been developed. FDSS/μCELL is capable of detecting messengers, such as Ca2+ and cAMP, which are major contributors to the GPCR signaling system by using fluorescence and luminescence probes. FDSS/μCELL allows simultaneous dispensing and kinetic measurement of compounds in whole microplate wells, thus realizing high throughput screening.
 ・Ca2+ measurement: Fluo-4, Fluo-8, Cal-520, Aequorin
 ・cAMP measurement: Glo-Sensor

 

2. Ionchannel

Ion channel, a class of transmembrane proteins that allow certain ions to pass through the cellular biomembrane (in or out of the cell), regulate cellular functions and are involved in the development of cardiovascular, neurologic and metabolic diseases. FDSS/μCELL performs high throughput drug screening using voltage-sensitive fluorescent dyes or fluorescent indicators for different ions.
 ・Na+ measurement: ANG-2, Corona-Green, Corona-Red, Sodium-Green
 ・K+ measurement: FluxOR
 ・Cl- measurement: MEQ, MQAE, YFP
 ・Membrane potential measurement: FluoVolt, Di-8-ANEPPS, DiBAC4 (3)

 

3. Luminescence

The merits, i.e., high sensitivity and low noise, of assays using luminescence probes have led to the wide application of such assays in various luciferase assay systems and Ca2+ assays using aequorin. FDSS/μCELL simultaneously performs different assays using luminescence probes on a single microplate with the use of highly sensitive two-dimensional sensors (camera), allowing for high throughput screening without bothersome time lags after substrate addition.

 

4. BRET/FRET

Biosensors based on the principle of resonance energy transfer that use GFP (green fluorescence protein) or Luc (luciferase) are utilized as a tool to measure various intracellular signal transmissions including ionic concentrations and signaling molecular activities. FDSS/μCELL simultaneously performs BRET (bioluminescence resonance energy transfer) measurements, a luminescence-based approach, and FRET (fluorescence resonance energy transfer) measurements, a fluorescence-based approach, on a single microplate using highly sensitive two-dimensional sensors (camera) and an automatic filter changer.
  ・BRET: BRET1, BRET2, Nano-BRET
  ・FRET: C/Y FRET, VSP, Cameleon

 

5. iPS-cell

Various differentiated cells have recently been created from iPSC (induced pluripotent stem cell), and this increasingly allows for the conduct of cell-based assays using human-derived native cells. In particular, iPS Cardiotoxicity, iPS Neurotoxicity, and iPS Hepatotoxicity assessments have been increasingly performed as safety evaluation of compounds. FDSS performs high throughput toxicity screening.

 

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Specifications

Dispense (96-tip type) A10118-24  10 μL to 200 μL using A10118-24 
Dispense (384-tip type) A10118-26  1 μL to 30 μL using A10118-26 
Sensor (ImagEM)  High-speed, high-sensitivity digital EM-CCD camera for fluorescence and luminescence 
Sampling rate  10 Hz (10 data point per second)
200 Hz (200 data point per second) maximum with U8524-11 option *1> 
Sampling interval  0.1 s to 100 s interval
0.005 s to 100 s with U8524-11 option *1 
Light source (L11601-06)  470 nm excitation and 540 nm emission
530 nm excitation and 593 nm emission 
Plate positions  One stage for assay plate, two stages for compound plate 
Adaptable microplate  Clear bottom black 96/384 plates (SBS format height 8 mm to 15 mm) 
Tip/Plate loading  Manual loading 
Number of sampling data point  1 to 50 000 samples 
Power supply specification  Input power supply: AC 100 V to AC 240 V, Frequency: 50 Hz/60 Hz 
Power consumption when AC 100 V to AC 120 V
(Data analysis unit and FDSS/μCELL main unit with heater) 
Approx. 1170 VA
(Data analysis unit: approx. 500 VA, dispenser main unit: approx. 300 VA, heater unit, approx. 370 VA) 
Ambient operating temperature  +15 ºC to +30 ºC 
Dimension/weight
Main unit (Data analysis unit is not included) 
550 mm (W)×670 mm (D)×1600 mm (H)/about 200 kg 
*1 FDSS software option: High speed data capture when using U8524-11

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Dimensions

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Related information

FDSS Application Note No.2 CoroNa Red as a potential probe for intracellular sodium imaging [0.1MB/PDF]

FDSS Application Note No.3 FRET-based Voltage Sensor dyes for Drug Screening [0.1MB/PDF]

FDSS Application Note No.6 Direct comparison of Fast response and Slow response Membrane potential dye using the FDSS6000 [0.1MB/PDF]

FDSS Application Note No.8 Comparison of No Wash Reagent Kits on the FDSS6000 [0.4MB/PDF]

FDSS Application Note No.11 Detection and Modulation of Cardiomyocyte Calcium Oscillations Using the FDSS6000 [0.1MB/PDF]

FDSS Application Note No.14 Evaluation of a new intracellular calcium fluorescent reagent using the FDSS6000 [0.4MB/PDF]

FDSS Application Note No.16 FluxORTM Differentially Identifies K+ Channel Antagonists Using FDSS6000 [0.1MB/PDF]

FDSS Application Note No.17 Use of Ca2+ oscillations in primary neuron culture: a new HTS model for neuronal diseases [0.3MB/PDF]

FDSS Application Note No.18 β-arrestin recruitment for GPCR Screening [0.1MB/PDF]

FDSS Application Note No.20 New Tools for Drug Discovery: Multiplexing the monitoring of Ca2+ and cAMP signaling in primary cells using the FDSS/μCell. [0.4MB/PDF]

FDSS Application Note No.22 High Speed Data Acquisition Option for FDSS series [0.4MB/PDF]

FDSS Application Note No.23 BRET1-assay using the FDSS/μCELL imaging plate reader [0.1MB/PDF]

FDSS Application Note No.24 Evaluation of membrane potential assay kit using the FDSS/μCELL imaging plate reader: Detection ion channel in nicotinic acetylcholine receptors (nAChRs) [0.1MB/PDF]

FDSS Application Note No.25 Identification of small molecule modulators of reactive oxygen release in TNF-α primed primary human neutrophils. [0.1MB/PDF]

FDSS Application Note No.26 BRET2 assay using the FDSS/μCELL: monitoring cell surface-receptor internalization [0.1MB/PDF]

FDSS Application Note No.27 Kinetic Luminescent Measurement of ROS (Reactive Oxygen Species) production with ABEL Cell activation ROS kit with PHOLASIN on the FDSS/μCELL platform [0.2MB/PDF]

FDSS Option 2: High Speed Acquisition Option [0.2MB/PDF]

FDSS Option 3: EFS pacing kit for FDSS/μCELL [0.3MB/PDF]

FDSS Option 4: EFS pacing kit for FDSS/μCELL [0.4MB/PDF]

FDSS/μCELL Cardiomyocyte in-vitro Toxicity Assay [Axiogenesis ver.] [1.4MB/PDF]

FDSS/μCELL Cardiomyocyte in-vitro Toxicity Assay [CDI ver.] [1.2MB/PDF]

FDSS/μCELL Cardiomyocyte in-vitro Toxicity Assay [ReproCELL ver.] [1.2MB/PDF]

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Publications

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