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GPCR | Kinetic Plate Imager
G-protein-coupled receptors (GPCRs) research and screening are areas that benefit the most from the FDSS kinetic plate imager’s high throughput feature. The FDSS can be equipped with 96-, 384- (FDSS/μCELL and FDSS-GX) and 1536- (FDSS-GX) manifold pipettors to add ligands / compounds into all wells simultaneously, and it images the whole plate so all wells are read at the same time, resulting in no time lag among wells while the reactions of cells occur. GPCRs play a major role in cell signaling, and many GPCR-targeted medical drugs have been developed. Applications (and resources) include:
- Traditional fluorescent non wash dyes, such as Fluo2 / Fluo4 / Fluo8 and protein based such as SECI
- Ratio dyes such as Fura2 or Cameleon
- Luminescence probes such as Aequorin, cAMP, BRET
- Multiplexing in fluorescence or luminescence
- Duplex assay: ELRIG poster, Webinar: Duplexed Screening of GPCRs to Measure Calcium and β-Arrestin
Related scientific material
- Publications
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Authors Title Source Le Poul E, Hisada S, Mizuguchi Y, Dupriez VJ, Burgeon E, Detheux M. Adaptation of aequorin functional assay to high throughput screening J Biomol Screen. 2002 Feb;7(1):57-65. Inbe H, Watanabe S, Miyawaki M, Tanabe E, Encinas JA. Identification and characterization of a cell-surface receptor, P2Y15, for AMP and adenosine J Biol Chem. 2004 May 7;279(19):19790-9. Epub 2004 Mar 4. McNamara CR, Mandel-Brehm J, Bautista DM, Siemens J, Deranian KL, Zhao M, Hayward NJ, Chong JA, Julius D, Moran MM, Fanger CM. TRPA1 mediates formalin-induced pain http://www.ncbi.nlm.nih.gov/pubmed/17686976 Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W. Identifying nonselective hits from a homogeneous calcium assay screen J Biomol Screen. 2007 Mar;12(2):285-7. Epub 2007 Feb 8. Wise H, Abel PW, Cawkill D. Use of reduced temperature cell pausing to enhance flexibility of cell-based assays J Biomol Screen. 2009 Jul;14(6):716-22. Epub 2009 May 21. Lin Tian, S. Andrews Hires, Tianyi Mao, Daniel Huber, M. Eugenia Chiappe, Sreekanth H. Chalasani, Leopoldo Petreanu, Jasper Akerboom, Sean A. McKinney, Eric R. Schreiter, Cornelia I. Bargmann, Vivek Jayaraman, Karel Svoboda and Loren L. Looger Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators Nature Methods 6, 875 - 881 (2009), online: 8 November 2009 | doi:10.1038/nmeth.1398 Pantel J, Williams SY, Mi D, Sebag J, Corbin JD, Weaver CD, Cone RD. Development of a high throughput screen for allosteric modulators of melanocortin-4 receptor signaling using a real time cAMP assay Eur J Pharmacol. 2011 Jun 11;660(1):139-47. Epub 2011 Feb 4. Jasper Akerboom, Tsai-Wen Chen, Trevor J. Wardill, Lin Tian, Jonathan S. Marvin, Sevinç Mutlu, Nicole Carreras Calderón, Federico Esposti, Bart G. Borghuis, Xiaonan Richard Sun, Andrew Gordus, Michael B. Orger, Ruben Portugues, Florian Engert, John J. Macklin, Alessandro Filosa, Aman Aggarwal, Rex A. Kerr, Ryousuke Takagi, Sebastian Kracun, Eiji Shigetomi, Baljit S. Khakh, Herwig Baier, Leon Lagnado, Samuel S.-H. Wang, Cornelia I. Bargmann, Bruce E. Kimmel, Vivek Jayaraman, Karel Svoboda, Douglas S. Kim, Eric R. Schreiter and Loren L. Looger Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging This article is freely available online through the J Neurosci Open Choice option. August 1, 2012 Olaposi I. Omotuyi, Jun Nagai & Hiroshi Ueda Lys39-Lysophosphatidate Carbonyl Oxygen Interaction Locks LPA1 N-terminal Cap to the Orthosteric Site and partners Arg124 During Receptor Activation Scientific Reports 5, Article number: 13343 (2015)doi:10.1038/srep13343 - Application Notes
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App. Note N°. Title FDSS Application Note N°. 14 FDSS Application Note No.14 Evaluation of a new intracellular calcium fluorescent reagent using the FDSS6000 [0.4MB/PDF] FDSS Application Note N°. 20 FDSS Application Note No.20 New Tools for Drug Discovery: Multiplexing the monitoring of Ca2+ and cAMP signaling in primary cells using the FDSS/μCell. [0.4MB/PDF] FDSS Application Note N°. 23 FDSS Application Note No.23 BRET1-assay using the FDSS/µCELL imaging plate reader [0.13MB/PDF] - Please note that information in posters and presentations used in past conferences and symposiums may now be slightly different. As Hamamatsu are solely an equipment manufacturer, we are not responsible for measurement samples or data presented in these materials.
- Related Scientific Materials from Users Meetings in the past
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- Confirmation
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