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Polymerase chain reaction (PCR) is a technique used in molecular biology to make millions of coplies of DNA. PCR is now a common and used in clinical and research laboratories for a broad variety of applications.Real-time PCR monitors the process of amplification in real time using fluorescence; not only at the end of the reaction as occurs in conventional PCR.
Digital PCR is a quantitative method that provides a sensitive way of measuring the amount of DNA in a sample.Photomultiplier tube (PMT), SiPM(MPPC) and CMOS camera are mainly considered for the fluorescence detection system.

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