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Biophotonics
3D fluorescene microscopy
Our goal is the development of microscopy for high-speed volumetric imaging to study the mechanisms of intracellular life activity. We are developing an effective imaging technique called light sheet fluorescence microscopy. It scans through the samples using a sheet-like excitation light to get series of 2D images and achieves volumetric imaging by combining these 2D images. This microscope makes it possible to observe cell-to-cell communication inside spheroids. For the purpose of finding undiscovered cell dynamics, we are committed to create higher-speed and higher-resolution 3D microscope with our optical technology.
Development of biological sensor device with microfluidic MEMS device
Our aim is to create a living device that brings out the functions of biological systems featuring high sensitivity and selectivity as well as saving energy, while compact and lightweight.
The world’s smallest "イ" made of genetic material, DNA
The hydrogen bonds of base pairs (A/T, G/C) are disrupted by heating. The complementary bases, however, connect again by cooling. Using this property, nanostructures can be made by using DNA as a material. Modifications of staple DNA (single-stranded DNA to assemble certain nanostructures) or tweaking of the A, T, G, C sequences enable the placement of chemical substances, proteins, double helices, etc., to any position in DNA structures with nanometer precision.
Photosynthesis evaluation technology by photon detection
Photosynthesis bio organisms system, that is plants and algae, are emitting ultra weak luminescence as kind of reverse reaction of photosynthetic energy. This called "delayed fluorescece". The ultra weak luminescence can be detected as to single photon. Each photon reflect chemical energy on single molecure that associated in the photosynthesis reaction. Kinetics of the photon emission, intensities and time behaviours, can reveal activity of photosynthesis reaction. This can be a novel technique to evaluate growth and qualities of plants and algae on enviromnental monitoring, and breeding.
Two-photon microscope using ultra-short pulsed laser
The femtosecond pulsed laser is made highly cost-effective by manufacturing its main parts in-house. We apply the laser to two-photon-excited luminescence imaging. Since both the oscillator and the amplifier sections are based on polarization maintaining optical fiber lasers, the femtosecond pulsed laser features high stability, reduced space for optical transmission, and compact size.
Optical sensing for agriculture
We are developing optical technology to improve agricultural operations and increase crop quality. In our plant condition diagnosis, the contents in the crops are investigated by spectral measurement in the range of visible/IR to estimate the optimum amount of fertilizer, predict harvest time and evaluate the crops quality.
To evaluate plant growth, nitrate ion concentration is monitored by measuring light absorbance of nutrient solution in UV range (approx. 210 nm). By measuring nitrate concentration and nutrient solution weights simultaneously, the amount of nitrate absorbed by the plant and the optimum amount of fertilizer are estimated.
Microbial detection and growth control by light
Autofluorescence makes it possible to detect fungus. Autofluorescence wavelength differs depending on bacterial species. The spectrum suggests the possibility of bacterial species identification. We can expect development in the fields of agriculture, food hygiene and medical care.
And we discovered visible light growth inhibition of denitrifying bacteria that occurs under anaerobic denitrification conditions. The important point is that under oxygen breathing conditions this inhibition is not observed. Application development to fermentation control of microorganisms, drug discovery, etc. is expected.