RESEARCH & DEVELOPMENTWith decades of accumulated expertise in the development of photonic technologies to rely upon, Hamamatsu Photonics conducts basic research to discover new knowledge and create new industries in the elds of biotechnology, medicine, information technology, communications, energy, materials, astronomy, and agriculture. We also strive to continuously develop exciting new products and enhance existing products with added functionality. In scal year 2018, funds allocated to research and development totaled JPY 12,830 million, an increase of 9.0% over the previous scal year. Some highlights of our R&D efforts are presented here. Basic researchPromoting research on biological imaging using wavefront control techniqueIn the optical information processing sector, we are pursuing research on in vivo imaging using wavefront control. With the recent advancement in genetic modication techniques and information processing techniques, three-dimensional in vivo observation is given greater importance in various led. While two-photon excitation uorescence microscopy (TPM) is known as an effective imaging method(*1), it is unable to provide clear intravital deep imaging due to optical aberrations generated from the samples themselves. Given these conditions, based on the wavefront control technology cultivated by the Company, we developed a new calculation method to correct aberration using parameters such as the surface shape of the sample, and applied it to the TPM equipped with our integrated spatial light modulator to successfully acquire clear deep imaging inside the sample(*2). While conventional calculation methods only allowed aberration corrections for samples with at surfaces, the new method enables the application of aberration corrections to samples with curved surfaces. These results are expected to contribute to intravital deep observation, as well as to be applied in various elds including neuroscience, regenerative medicine and next-generation laser processing.(*1)This method observes the uorescence by exciting uorescent molecules from the simultaneous absorption of two photons. Because near-infrared light with greater intravital penetration can be used, it allows observing of deeper areas compared to conventional uorescence microscopy.(*2)Results of this research was achieved through joint research with the Hamamatsu University School of Medicine.Blood vessels in a mouse cerebrum observed by TPM. Deep region of the sample is clearly observed in the images (A1, A2) acquired with the novel method compared to the images (B1, B2) acquired with a conventional method. (1μm=1/1000mm)A1B10μm500μm1,000μm1,500μm2,000μmA2B2It is indicated that the closerto white the color is, the strongerthe fluorescence is.8


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