CMOS cameras CMOS cameras

Publications | sCMOS cameras

Publications

ORCA-Fusion BT
Authors Title Source
Pratish Thakore, Michael G Alvarado, Sher Ali, Amreen Mughal, Paulo W Pires, Evan Yamasaki, Harry AT Pritchard, Brant E Isakson, Cam Ha T Tran, Scott Earley Brain endothelial cell TRPA1 channels initiate neurovascular coupling eLife 10, e63040 (2021).
Lexy von Diezmann and Ofer Rog Single-Molecule Tracking of Chromatin-Associated Proteins in the C. elegans Gonad J. Phys. Chem. B 2021, 125, 23, 6162–6170
Kei Yamamoto, Haruko Miura, Motohiko Ishida, Yusuke Mii, Noriyuki Kinoshita, Shinji Takada, Naoto Ueno, Satoshi Sawai, Yohei Kondo & Kazuhiro Aoki Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis Nat Commun 12, 7145 (2021).
Mbaye Diouf, Mitchell Harling, Murat Yessenov, Layton A. Hall, Ayman F. Abouraddy, and Kimani C. Toussaint, Space-time vector light sheets Opt. Express 29, 37225-37233 (2021)
Oliver Klatt, Daniele Repetto, Johannes Brockhaus, Carsten Reissner, Abderazzaq El khallouqi, Astrid Rohlmann, Martin Heine, Markus Missler, Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior Cell Reports, Volume 35, Issue 11, 2021, 109266,
Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto, Takashi Ito Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae G3 Genes|Genomes|Genetics, Volume 11, Issue 12, December 2021, jkab304

ORCA-Fusion
Authors Title Source
Piatkevich, K.D., Bensussen, S., Tseng, Ha. et al. Population imaging of neural activity in awake behaving mice Nature 574, 413–417 (2019).
Silvia Benito-Kwiecinski, Stefano L. Giandomenico, Magdalena Sutcliffe, Erlend S. Riis, Paula Freire-Pritchett, Iva Kelava, Stephanie Wunderlich, Ulrich Martin, Gregory A. Wray, Kate McDole, Madeline A. Lancaster An early cell shape transition drives evolutionary expansion of the human forebrain Cell, Volume 184, Issue 8, 2021, Pages 2084-2102.e19,
Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini-Nassab, Michael F. Z. Wang, David McKellar, Hao Shi, Benjamin Grodner, Jonathan T. Butcher & Iwijn De Vlaminck Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis Nat Commun 12, 1771 (2021).
Kiryl D. Piatkevich, Seth Bensussen, Hua-an Tseng, Sanaya N. Shroff, Violeta Gisselle Lopez-Huerta, Demian Park, Erica E. Jung, Or A. Shemesh, Christoph Straub, Howard J. Gritton, Michael F. Romano, Emma Costa, Bernardo L. Sabatini, Zhanyan Fu, Edward S. Boyden & Xue Han Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy Nat Biotechnol 39, 609–618 (2021).
Xinyu Wang, Chong Liu, Siwei Zhang, Huiwen Yan, Liwen Zhang, Amin Jiang, Yong Liu, Yun Feng, Di Li, Yuting Guo, Xinyao Hu, Yajing Lin, Pengcheng Bu, Dong Li, N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles Developmental Cell, Volume 56, Issue 5, 2021, Pages 702-715.e8,
Emily C. Wheeler, Anthony Q. Vu, Jaclyn M. Einstein, Matthew DiSalvo, Noorsher Ahmed, Eric L. Van Nostrand, Alexander A. Shishkin, Wenhao Jin, Nancy L. Allbritton & Gene W. Yeo Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors Nature Methods volume 17, pages636–642 (2020)
Scott M. Emrich, Ryan E. Yoast, Ping Xin, Vikas Arige, Larry E. Wagner, Nadine Hempel, Donald L. Gill, James Sneyd, David I. Yule, Mohamed Trebak, Omnitemporal choreographies of all five STIM/Orai and IP3Rs underlie the complexity of mammalian Ca2+ signaling Cell Reports, Volume 34, Issue 9, 2021, 108760,
Tomomi Suzuki, Tetsuo Mioka, Kazuma Tanaka & Akira Nagatani An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast Sci Rep 10, 12474 (2020).
Nicholas S. Groves,Merissa M. Bruns and Schuyler B. van Engelenburg A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly Pathogens 2020, 9(11), 972
Pierre Stömmer, Henrik Kiefer, Enzo Kopperger, Maximilian N. Honemann, Massimo Kube, Friedrich C. Simmel, Roland R. Netz & Hendrik Dietz A synthetic tubular molecular transport system Nat Commun 12, 4393 (2021).
Hugh Sparks, Lucas Dent, Chris Bakal, Axel Behrens, Guillaume Salbreux, and Chris Dunsby Dual-view oblique plane microscopy (dOPM) Biomed. Opt. Express 11, 7204-7220 (2020)
Philipp Zelger, Lisa Bodner, Martin Offterdinger, Lukas Velas, Gerhard J. Schütz, and Alexander Jesacher Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence Biomed. Opt. Express 12, 802-822 (2021)

ORCA-Flash4.0
Authors Title Source
Xuanze Chen, Zhiping Zeng, Rongqin Li, Boxin Xue, Peng Xi, Yujie Sun Superior performance with sCMOS over EMCCD in super-resolution optical fluctuation imaging Journal of Biomedical Optics
Luchang Li, Mengting Li, Zhaoning Zhang, Zhen-Li Huang Assessing low-light cameras with photon transfer curve method Journal of Innovative Optical Health Sciences
S Hayashi, Y Okada Ultrafast superresolution fluorescence imaging with spinning disk confocal microscope optics Molecular Biology of the Cell
Matthias Mehling, Tino Frank, Cem Albayrak, Sava? Tay Real-time tracking, retrieval and gene expression analysis of migrating human T cells Lab on a Chip
Ying S. Hu, Maxwell Zimmerley, Yu Li, Robin Watters, and Hu Cang Single-Molecule Super-Resolution Light-Sheet Microscopy ChemPhysChem
Peter W Winter and Hari Shroff Faster fluorescence microscopy: advances in high speed biological imaging Current Opinion in Chemical Biology
Ronny Forster, Hui-Wen Lu-Walther, Aurelie Jost, Martin Kielhorn, Kai Wicker, and Rainer Heintzmann Simple structured illumination microscope setup with high acquisition speed by using a spatial light modulator Optics express
Jeffrey P. Nguyen, Frederick B. Shipley, Ashley N. Linder, George S. Plummer, Joshua W. Shaevitz, and Andrew M. Leifer Whole-brain calcium imaging with cellular resolution in freely behaving C. elegans Neurons and Cognition
Zong W, Zhao J, Chen X, Lin Y, Ren H, Zhang Y, Fan M, Zhou Z, Cheng H, Sun Y, Chen L. Large-field high-resolution two-photon digital scanned light-sheet microscopy  Cell Res. 2014 Sep 26. doi: 10.1038/cr.2014.124. [Epub ahead of print]
Vladimirov N, Mu Y, Kawashima T, Bennett DV, Yang CT, Looger LL, Keller PJ, Freeman J, Ahrens MB. Light-sheet functional imaging in fictively behaving zebrafish Nat Methods. 2014 Jul 27. doi: 10.1038/nmeth.3040. [Epub ahead of print]
Mickoleit M, Schmid B, Weber M, Fahrbach FO, Hombach S, Reischauer S, Huisken J. High-resolution reconstruction of the beating zebrafish heart Nat Methods. 2014 Jul 20. doi: 10.1038/nmeth.3037. [Epub ahead of print]
Vettenburg T, Dalgarno HI, Nylk J, Coll-Lladó C, Ferrier DE, Čižmár T, Gunn-Moore FJ, Dholakia K. Light-sheet microscopy using an Airy beam Nat Methods. 2014 May;11(5):541-4. doi: 10.1038/nmeth.2922. Epub 2014 Apr 6.
Juette MF, Terry DS1, Wasserman MR, Zhou Z, Altman RB, Zheng Q, Blanchard SC. The bright future of single-molecule fluorescence imaging Curr Opin Chem Biol. 2014 Jun 20;20C:103-111. doi: 10.1016/j.cbpa.2014.05.010.
Menon M, Askinazi OL, Schafer DA. Dynamin2 organises lamellipodial actin networks to orchestrate lamellar actomyosin PLoS One. 2014 Apr 7;9(4):e94330. doi: 10.1371/journal.pone.0094330. eCollection 2014.
Gualda EJ, Vale T, Almada P, Feijó JA, Martins GG, Moreno N. OpenSpinMicroscopy: an open-source integrated microscopy platform Nat Methods. 2013 Jul;10(7):599-600. doi: 10.1038/nmeth.2508. Epub 2013 Jun 9.
Fahrbach FO, Voigt FF, Schmid B, Helmchen F, Huisken J. Rapid 3D light-sheet microscopy with a tunable lens Opt Express. 2013 Sep 9;21(18):21010-26. doi: 10.1364/OE.21.021010.
Fullerton S, Bennett K, Toda E, Takahashi T. Optimization of precision localization microscopy using CMOS camera technology Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280T (February 9, 2012); doi:10.1117/12.906336; http://dx.doi.org/10.1117/12.906336
Fullerton S, Bennett K, Toda E, Takahashi T. Camera simulation engine enables efficient system optimization for super-resolution imaging Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 822811 (February 9, 2012); doi:10.1117/12.906346; http://dx.doi.org/10.1117/12.906346
Singh AP, Krieger JW, Buchholz J, Charbon E, Langowski J, Wohland T. The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy. Opt Express. 2013 Apr 8;21(7):8652-68.
Ahrens MB, Keller PJ. Whole-brain functional imaging at cellular resolution using light-sheet microscopy.  Nat Methods. 2013 Mar 18. doi: 10.1038/nmeth.2434.
Long F, Zeng S, Huang ZL. Localization-based super-resolution microscopy with an sCMOS camera part II: experimentalmethodology for comparing sCMOS with EMCCD cameras. Opt Express. 2012 Jul 30;20(16):17741-59.
Baumgart E, Kubitscheck U. Scanned light sheet microscopy with confocal slit detection. Opt Express. 2012 Sep 10;20(19):21805-14.
Takao D, Nemoto T, Abe T, Kiyonari H, Kajiura-Kobayashi H, Shiratori H, Nonaka S. Asymmetric distribution of dynamic calcium signals in the node of mouse embryo during left-right axis formation. Dev Biol. 2013 Apr 1;376(1):23-30. 
Huang F, Hartwich TM, Rivera-Molina FE, Lin Y, Duim WC, Long JJ, Uchil PD, Myers JR, Baird MA, Mothes W, Davidson MW, Toomre D, Bewersdorf J. Video-rate nanoscopy using sCMOS camera-specific single-molecule localization algorithms. Nat Methods. 2013 May 26. doi: 10.1038/nmeth.2488.
Ma H, Kawai H, Toda E, Zeng, S, Huang ZL Localization-based super-resolution microscopy with an sCMOS camera part III: camera embedded data processing significantly reduces the challenges of massive data handling Optics Letters, Vol. 38, Issue 11, pp. 1769-1771 (2013)
Baumgart E., Kaminski T. Scanned LSFM with Confocal Detection. Imaging and Microscopy, Volume 15, Issue 1 2013.
Xu D, Jiang T, Li A, Hu B, Feng Z, Gong H, Zeng S, Luo Q. Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device. J Biomed Opt. 2013 Jun;18(6):60503. doi: 10.1117/1.JBO.18.6.060503.

Special site

This site provides information on scientific cameras.

Since there is a wide range of camera types and performance, it is important to select the best camera for each application.

It introduces technical information, simulation tools, and examples of actual applications to help you fully understand the performance of the camera and select the best one for your application.