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Q&A | 転写プレート Poropare™
基本特性
| Poropare™の具体的な構成は？ | Poropare™は約50 μmのガラス粒子によって構成された多孔プレートを母材として、その有効面にPtのイオン化支援層を有しています。 |
| Poropare™の外形および有効面のサイズは？ | Poropare™のサイズは52 mm ✕ 16 mm、有効面のサイズはプレート中心から47 mm ✕ 11 mm以上となります。最外周部までPt膜が成膜されていますが、これはアダプター固定時の導通確保のためです。 |
| マトリックスフリーで、どのくらいの分子量まで検出できますか？ | サンプルによりますが、m/z 2000程度まではポリエチレングリコール等の高分子材料で検出された実績があります。なお、タンパク質などの高分子の検出は困難です。 |
| イメージング測定での空間分解能はどのくらいですか？ | 質量分析装置の性能や測定条件にもよりますが、Poropare™の構造上、50 μmピッチが空間分解能の限界と考えられます。含水量の多いサンプルを転写する場合は、にじみが空間分解能に影響します。 |
| Poropare™を用いた質量分析の結果に特徴はありますか？ | プロトン付加が検出されない場合は、Na、K付加として検出されることがあります。特に、Naイオンが付加しやすい傾向にあります。 |
| イメージングではなく、一般的なSALDI基板のような使い方（滴下測定）はできますか？ | 可能です。ただし、Poropare™内部への浸透がありますので、一般的な基板のような表面での濃縮が困難です。 |
| 繰り返し使えますか？ | MALDI-TOFMSで測定すると、測定箇所でPtのアブレーションが発生します。Pt膜が消失すると必要な機能が失われますので、使用は1回限りとなります。 |
購入・サービス
| Poropare™および専用アダプターはどこで購入できますか？ | 現在、日本電子株式会社よりご購入いただけます。（お問い合わせフォーム） 詳細につきましては日本電子株式会社のお薦め消耗品サイト をご参照ください。 |
| Poropare™はどこで試せますか？ | 現在は、株式会社プレッパーズでの受託分析でお試しいただけます。なお、試用環境は今後拡大していく予定です。 株式会社プレッパーズ |
| 日本国外での販売は予定されていますか？ | 現在は、日本国内での販売のみとなっております。海外での販売は準備が整い次第実施する予定です。 |
| 販売中の仕様と異なるタイプ（サイズ違い、学会発表時の仕様など）は入手可能ですか？ | 現在、販売可能な製品はラインナップ品のみとなります。外部発表の一部には、開発品が使用された結果を含みますのでご了承ください。カスタマイズのご希望はこちらよりご相談ください。少数でのご希望はお受けできない場合もありますが、今後の製品展開の参考にさせていただきます。 |
| Poropare™に関する新規ビジネス、共同開発等は受け付けますか？ | Poropare™の普及に資する事業およびビジネスパートナーを随時受け付けます。こちらよりご相談ください。 |
使用環境について
| 推奨のMALDI-TOFMS装置はありますか？ | 日本電子株式会社製のJMS-S3000 SpiralTOF™シリーズでの使用を推奨します。Poropare™専用のアダプターが用意されており、Poropare™の使用が認められています。 |
| 推奨のMALDI-TOFMS装置以外でも使用できますか？ | 推奨装置以外での使用については、各装置製造元にご相談ください。ただし、Poropare™の使用による障害等について当社は一切責任を負いません。 |
| Poropare™の使用にあたって必要な付属品はありますか？ | Poropare™の使用には専用アダプターと、固定及びアダプターとの導通確保のために導電性テープ・シール（カーボンテープ等）が必要です。 |
| 専用アダプターとはどういったものですか？ | Poropare™のサイズに合った凹部を有するアダプターで、JMS-S3000 SpiralTOF™シリーズ用の専用アダプターが日本電子株式会社より販売されております。 |
使用方法について
| 転写に適しているサンプルはどのようなものですか？ | 水分を含むサンプル（植物等）はPoropare™のもつ吸収性から転写に適しています。また、適度な柔らかさをもつサンプル（樹脂、ゴム等）も、有効面に接触させやすい傾向があります。適性はサンプル毎に異なりますので、上記の限りではないことをご承知ください。 |
| 水分を含まないサンプルでも転写できますか？ | 可能です。フィルム上の乾燥シミを転写した例があります。 |
| 物理接触だけでは転写が難しい場合は？ | Poropare™上での溶媒抽出が効果的です。あらかじめPoropare™に溶媒を含ませて転写することで、転写効率が向上した例があります。ただし、同時に空間分解能が低下する点にご注意ください。 |
〇〇は測定できますか？ どうすれば測定できますか？ 等のご質問について |
目的の結果を得るまでには、Poropare™だけでなく装置設定も含んだ包括的な検討が必要な場合もあります。弊社では、個々のサンプルについての測定サポートはいたしかねます。 |
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