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Immunochromato-Reader

Immunochromatographic technique | Immunochromato Reader (Lateral flow reader)

 

Immunochromatography is a testing method for detecting a disease by dropping the sample containing an analyte onto a test strip. This is a speedy and simple technique that produces diagnostic results in 10 to 15 minutes after dropping the sample. An allergic reaction indicating a positive test result appears as a colored line.

Tests results from using immunochromatography are usually judged by a visual check, but using a high-sensitivity reader can find a disease with good accuracy even when the colored line is very thin. Since the intensity of the colored line is proportional to the amount of analyte in the sample, increasing the reader’s sensitivity gives good test results from just a small amount of sample which lowers the burden on the person being tested.


Measurement techniques by good points

Visual inspection

Ordinary measurement technique by immunochromatography

Reader (Absorption method for measuring color intensity)

Typical measurement technique when using a reader.

Reader (Fluorescence method for measuring fluorescence intensity)

Technique ideal for even small amounts of virus since detection sensitivity is high compared to absorption method.

  Visual inspection Testing by reader
Absorption method Fluorescence method
Sensitivity Low High Very high
Easy to handle Easy Not so easy
Time management Handring Auto
Reagent error detection Not detectable Reader can detect
Digitizing of reaction results Judgment by color chart Judgment by reader
Recording judgment results Requires a scanner or camera Auto

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