FDSS/μCELL Functional Drug Screening System
FDSS/μCELL is a laboratory screening system that compactly integrates technologies developed in drug discovery screening, enabling a purpose-built system that is simple to use.
Simultaneous measurement and analysis of the kinetics of a sample’s fluorescence or luminescence intensity in all wells at the time of compound addition are made possible by the high sensitivity two-dimensional sensor (camera) and dispenser head (96 tip type / 384 tip type). Screening various compounds at high throughput is enabled by measurement under the same conditions with no time lag between wells.
GPCRs (G protein-coupled receptors) play a major role in cell signaling, and many GPCR-targeted medical drugs have been developed. FDSS/μCELL is capable of detecting messengers, such as Ca2+ and cAMP, which are major contributors to the GPCR signaling system by using fluorescence and luminescence probes. FDSS/μCELL allows simultaneous dispensing and kinetic measurement of compounds in whole microplate wells, thus realizing high throughput screening.
・Ca2+ measurement: Fluo-4, Fluo-8, Cal-520, Aequorin
・cAMP measurement: Glo-Sensor
Ion channel, a class of transmembrane proteins that allow certain ions to pass through the cellular biomembrane (in or out of the cell), regulate cellular functions and are involved in the development of cardiovascular, neurologic and metabolic diseases. FDSS/μCELL performs high throughput drug screening using voltage-sensitive fluorescent dyes or fluorescent indicators for different ions.
・Na+ measurement: ANG-2, Corona-Green, Corona-Red, Sodium-Green
・K+ measurement: FluxOR
・Cl- measurement: MEQ, MQAE, YFP
・Membrane potential measurement: FluoVolt, Di-8-ANEPPS, DiBAC4 (3)
The merits, i.e., high sensitivity and low noise, of assays using luminescence probes have led to the wide application of such assays in various luciferase assay systems and Ca2+ assays using aequorin. FDSS/μCELL simultaneously performs different assays using luminescence probes on a single microplate with the use of highly sensitive two-dimensional sensors (camera), allowing for high throughput screening without bothersome time lags after substrate addition.
Biosensors based on the principle of resonance energy transfer that use GFP (green fluorescence protein) or Luc (luciferase) are utilized as a tool to measure various intracellular signal transmissions including ionic concentrations and signaling molecular activities. FDSS/μCELL simultaneously performs BRET (bioluminescence resonance energy transfer) measurements, a luminescence-based approach, and FRET (fluorescence resonance energy transfer) measurements, a fluorescence-based approach, on a single microplate using highly sensitive two-dimensional sensors (camera) and an automatic filter changer.
・BRET: BRET1, BRET2, Nano-BRET
・FRET: C/Y FRET, VSP, Cameleon
Various differentiated cells have recently been created from iPSC (induced pluripotent stem cell), and this increasingly allows for the conduct of cell-based assays using human-derived native cells. In particular, iPS Cardiotoxicity, iPS Neurotoxicity, and iPS Hepatotoxicity assessments have been increasingly performed as safety evaluation of compounds. FDSS performs high throughput toxicity screening.
Additionally, the optional EFS head pacing system enables new applications of the FDSS platform using cardiomyocytes, skeletal muscle cells, and neurons.
|Dispense (96-tip type) A10118-24||10 μL to 200 μL|
|Dispense (384-tip type) A10118-26||1 μL to 30 μL|
|Sensor (ImagEM)||High-speed, high-sensitivity digital EM-CCD camera for fluorescence and luminescence|
|Sampling rate||10 Hz (10 data point per second)|
200 Hz (200 data point per second) maximum with U8524-11 option
|Sampling interval||0.1 s to 100 s interval|
0.005 s to 100 s interval with U8524-11 option
|Light source (L11601-06)||470 nm excitation and 540 nm emission|
530 nm excitation and 593 nm emission
|Plate positions||One stage for assay plate, two stages for compound plate|
|Adaptable microplate||Clear bottom black 96/384 plates (SBS format height 8 mm to 15 mm)|
|Tip/Plate loading||Manual loading|
|Number of sampling data point||1 to 50 000 sampling|
|Power supply specification||Input power supply: AC 100 V to AC 240 V, Frequency: 50 Hz/60 Hz|
|Power consumption when AC 100 V to AC 120 V|
(Data analysis unit and FDSS/μCELL main unit with heater)
|Approx. 1300 VA|
(Data analysis unit: approx. 500 VA, dispenser main unit: approx. 300 VA, heater unit, approx. 500 VA)
|Power consumption when AC 200 V to AC 240 V|
(Data analysis unit and FDSS/μCELL main unit with heater)
|Approx. 1170 VA|
(Data analysis unit: approx. 500 VA, dispenser main unit: approx. 300 VA, heater unit, approx. 370 VA)
|Ambient operating temperature||+15 ºC to +30 ºC|
Main unit (Data analysis unit is not included)
|550 mm (W)×675 mm (D)×1600 mm (H)/approx. 200 kg|
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