ORCA-Fusion BT Digital CMOS camera

C15440-20UP

See what you’ve been missing

The ORCA-Fusion BT camera is the pinnacle of scientific CMOS (sCMOS) performance. The specifications are without compromise: ultra-low readout noise, CCD-like uniformity, fast frame rates and back-thinned enabled high QE. But the beauty of the ORCA-Fusion BT is what this combination of exceptional photon detection and collection can do for you. See the dimmest whisper of signal, acquire visually stunning, high S/N images from the fewest photons, capture previously unresolved temporal events and perform computational methods with confidence. The ORCA-Fusion BT is the camera that makes the difficult experiments easy and previously impossible experiments possible.

Special sites

This site provides information on scientific cameras.
Since there is a wide range of camera types and performance, it is important to select the best camera for each application.
It introduces technical information, simulation tools, and examples of actual applications to help you fully understand the performance of the camera and select the best one for your application.

Special site for Scientific cameras

ORCA-Fusion BT advantages versus other BT CMOS cameras

Exclusive industry leading BT sCMOS performance with trusted Hamamatsu quality and reliability

  • Extremely low readout noise
  • High QE, across a wide range of wavelengths
  • CCD-like uniformity; minimal high readout noise pixels
  • High dynamic range
  • 3 readout speeds
  • Lightsheet readout mode
  • 5.3 Megapixels
  • Both USB 3.0 and high speed CoaXPress interfaces

ORCA-Fusion BT advantages versus EM-CCD cameras

A practical and functional replacement for expensive EM-CCDs with uncompromising sensitivity, speed and versatility

  • Resolution
  • Binning versatility
  • No amplification or excess noise
  • High dynamic range
  • Large field of view

Applications

  • Wide-field fluorescence microscopy
  • Time-lapse imaging
  • Spinning disk confocal microscopy
  • Lightsheet microscopy
  • Super resolution precision localization
  • Genetically encoded voltage imaging
  • Optogenetics
  • Single molecule tracking
  • Computational imaging
  • Quantum computing

Time-lapse live cell imaging by low light excitation

Sample: H9c2, Objective lens: Apo TIRF 60XC (NA1.49), ND: 8, Interval: 15 sec

Nucleus

c15440-20up Application example 1

Nucleus:
HCS NuclearMask Stains / Exposure 20 ms

Mitochondria

c15440-20up Application example 3

Mitochondria:
MitoTracker / Exposure 500 ms

Membrane

c15440-20up Application example 2

Membrane:
CellMask Plasma Membrane stains / Exposure 500 ms

Lightsheet Readout Mode [Patented]

“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.

What is Lightsheet Readout Mode?

Lightsheet Readout Mode

PC recommendation

With the introduction of the ORCA-Fusion BT, users are now able to stream 5 megapixel images to their computers 89 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Fusion BT.

Software

Our software provides the interface to access all of our carefully engineered camera features, from simply setting exposure to orchestrating complex triggering for multidimensional experiments.

We have designed a Software API to be used by all Hamamatsu digital cameras, named DCAM-API, and designed a Software Development Kit for the integration of Hamamatsu cameras with custom software.

Third party software

As imaging setups become more complex, software has to control not only a camera, but also many other devices such as microscopes, stages and filter wheels… Therefore, companies have integrated Hamamatsu DCAM based cameras into their software products.

Specifications

Product number C15440-20UP
Imaging device Scientific CMOS image sensor
Effective number of pixels 2304 (H) × 2304 (V)
Pixel Size 6.5 μm × 6.5 μm
Effective Area 14.976 mm (H) × 14.976 mm (V)
Full well capacity 15 000 electrons *1
Readout speed Fast scan, Full resolution, CoaXPress : 89.1 frames/s
Fast scan, Full resolution, USB 3.0 : 31.6 frames/s(16 bit), 42.2 frames/s(12 bit) , 63.3 frames/s(8 bit)
Standard scan, Full resolution, CoaXPress : 23.2 frames/s
Standard scan, Full resolution, USB 3.0 : 23.2 frames/s
Ultra quiet scan, Full resolution, CoaXPress : 5.42 frames/s
Ultra quiet scan, Full resolution, USB 3.0 : 5.42 frames/s
Readout noise Fast scan : 1.6 electrons rms *1
Standard scan : 1.0 electrons rms *1
Ultra-quiet scan: 0.7 electrons rms *1
Exposure times Fast scan : 17 µs to 10 s
Standard scan : 65 µs to 10 s
Ultra quiet scan : 280 µs to 10 s
Cooling method Peltier cooling
Cooling temperature Forced-air cooled (Ambient temperature: +25 ˚C) : −8 ˚C
Water cooled (Water temperature: +25 ˚C) : −8 ˚C
Water cooled (Max cooling (Ambient temperature: +20 ˚C, water temperature: +20 ˚C)) : Less than −15 ˚C
Dark current 1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Ambient temperature: +25 ˚C)*1*3
1.0 electrons/pixel/s (cooling temperature: -8 ˚C , Water temperature: +25 ˚C)*1*3
0.7 electrons/pixel/s (cooling temperature: Less than −15 ˚C , Ambient temperature: +20 ˚C, water temperature: +20 ˚C)*1*3
Dynamic range 21 400:1 *1*2*4
External trigger mode Edge, Level, Sync readout, Start, Global reset edge, Global reset level, Programmable
External trigger signal routing SMA
External trigger delay function 0 μs to 10 s (1 μs step)
Trigger output Global exposure timing, Trigger ready, Low, High, 3 programmable timing outputs
External signal output routing SMA
Interface CoaXPress (Dual CXP-6) and USB 3.0 Super Speed *5
A/D converter 16 bit, 12 bit, 8 bit
Lens mount C-mount (standard), F-mount (C15440-20UP01)
Power supply AC 100 V to AC 240 V, 50 Hz/60 Hz
Power consumption Approx. 155 VA
Ambient operating temperature 0 °C to +40 °C
Ambient storage temperature -10 ℃ to +50 ℃
Ambient operating humidity 30 % to 80 % (With no condensation)
Ambient storage humidity 90 % or less (With no condensation)

*1 Typical value

*2 Calculated from the ratio of the full well capacity and the RMS read noise

*3 Dark current depends on cooling temperature

*4 In ultra-quiet scan

*5 Equivalent to USB 3.1 Gen 1 (SuperSpeed USB 5 Gbps)

Spectral response

Dimensions

Instruction manual

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