The L16462-01 is a light source integrating lamps and bundled optical fibers for diffuse reflection measurement in near-infrared spectrophotometry. This light from lamps is irradiated to a sample, and is diffused and reflected. This light from lamps is irradiated to a sample, and is diffused and reflected. The light which is guided into the optical fibers can be used for spectroscopic analysis by connecting with a near-infrared spectrometer. By arranging multiple lamps and optical fibers close to each other this light source can effectively detects the weak diffused light emitted from the sample.
Using the diffuse reflection light source L16462-01 and Hamamatsu’s near-infrared spectrometer FTIR engine, theophylline was quantitatively measured by PLS regression analysis*2 in 6 types of tablets including different theophylline concentrations.
Theophylline content is predicted by making a calibration curve from previously acquired training data*3 and doing PLS regression analysis.
The coefficient of determination on the calibration curve is R2=0.998, showing high accuracy measurements are possible.
*3: When quantifying a substance, this is data that expresses the change with respect to concentration by measuring absorbance of the standard substance in advance.
In two wavelength ranges, 1100 nm to 2000 nm and 2000 nm to 2500 nm, using a commercial light source and Hamamatsu’s diffuse reflection light source L16462-01, each theophylline concentration was measured by PLS regression analysis and accuracy was predicted as described above.
Typical commercial light sources have low quantification accuracy in the long wavelength range of 2000 nm and above. In contrast, Hamamatsu’s diffuse reflection light source has high photon detection efficiency in the near-infrared region up to 2500 nm, so compared to commercial light sources, it achieves high quantification accuracy even when measuring wavelengths of 2000 nm or above.
|PLS regression analysis||Commercial light source||NEW Diffuse reflection light source|
|1100 nm to 2000 nm||2000 nm to 2500 nm||1100 nm to 2000 nm||2000 nm to 2500 nm|
|Constant of determination (R2)||0.993||0.968||0.998||0.998|
|Root mean square deviation (RMSE)||0.0115||0.0144||0.0064||0.006|
|Mean absolute percentage error (MAPE)||5.60%||6.60%||2.10%||1.50%|
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