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FDSS®-μCELL v2 Kinetic Plate Imager
C13299-02
FDSS®-μCELL is a laboratory screening system that compactly integrates technologies developed in drug discovery screening, enabling a purpose-built system that is simple to use.
Simultaneous measurement and analysis of the kinetics of a sample’s fluorescence or luminescence intensity in all wells at the time of compound addition are made possible by the high sensitivity qCMOS® sensor and dispenser head (96 tip type / 384 tip type). Screening various compounds at high throughput is enabled by measurement under the same conditions with no time lag between wells.
FDSS is a registered trademark of Hamamatsu Photonics K.K. (China, France, Germany, Italy, Japan, UK, USA).
Achieving high-sensitivity imaging across a wide range of applications
High-sensitivity luminescence / high-speed fluorescence measurement
The high sensitivity qCMOS sensor comes as a standard in the FDSS-μCELL v2 optical system. In fluorescence and luminescence measurements, high quantitative performance has been achieved for low-light imaging and high-speed fluorescence changes. Even with fluorescent proteins that have low fluorescence intensity, high-precision real-time measurements can be achieved, enabling more detailed analysis of cellular responses.
Cell number evaluation using Cell Viability Assay kit
The qCMOS sensor offers measurement with wide linearity range and high sensitivity, enabling luminescence detection even with small numbers of cells.
Cell : CHO-K (15 000, 5000, 500, 150, 50, 15 cells/well)
Kit : Cell Titer (Promega Corporation)
Measurement of calcium concentration changes associated with beating in iPS-derived cardiomyocytes
qCMOS sensors enable high-speed measurement of minute changes in fluorescence.
Cell : iCell Cardiomyocytes2 (CDI)
Dye : EarlyTox kit
Condition : PROTAC Evaluation high speed, modeconc=0.1, 1, 10 µM, 48 h exposure
EFS pacing system
Electric field stimulation using electrodes is an effective technique for pacing of cardiomyocyte and skeletal muscle cells and for neuronal oscillation. FDSS-μCELL v2 simultaneously stimulates all wells of a 96 multi-EFS electrodes. It can be used in contraction timing control of muscle cells such as cardiomyocytes and skeletal muscle, or in Ca2+ oscillation control of nerve cells.
Example of measuring automatically beating iPS-derived cardiomyocytes
EFS pacing can be applied during calcium measurement (Cal-520AM) in automatically beating iPS-derived cardiomyocytes.
Cell : MyoridgeCarmyA
Dye : 2 μM Cal-520AM
Condition : 1st frequency 0.5 Hz, 2nd frequency 1 Hz
Applications
1. GPCR
FDSS-μCELL v2 is capable of detecting messengers, such as Ca2+ and cAMP, which are major contributors to the GPCR signaling system by using fluorescence and luminescence probes. FDSS-μCELL v2 allows simultaneous dispensing and kinetic measurement of compounds in whole microplate wells, thus realizing high throughput screening.
・Ca2+ measurement: Fluo-4, Fluo-8, Cal-520, Aequorin
・cAMP measurement: Glo-Sensor
2. Ion channel
FDSS-μCELL v2 performs high throughput drug screening using voltage-sensitive fluorescent dyes or fluorescent indicators for different ions.
・Na+ measurement: ANG-2, Corona-Green, Corona-Red, Sodium-Green
・K+ measurement: FluxOR
・Cl- measurement: MEQ, MQAE, YFP
・Membrane potential measurement: FluoVolt, Di-8-ANEPPS, DiBAC4 (3)
3. Luminescence
With its high-sensitivity qCMOS sensor, the FDSS-μCELL v2 performs different assays simultaneously using luminescence probes on a single microplate. This allows high-throughput screening without bothersome time lags after substrate addition.
4. BRET / FRET
FDSS-μCELL v2 simultaneously performs BRET (bioluminescence resonance energy transfer) measurements, a luminescence-based approach, and FRET (fluorescence resonance energy transfer) measurements, a fluorescence-based approach, on a single microplate using the high sensitivity qCMOS sensor and an automatic filter changer.
・BRET: BRET1, BRET2, NanoBRETⓇ
・FRET: C/Y FRET, VSP, Cameleon
5. iPS-cell
Various differentiated cells have recently been created from iPSC (induced pluripotent stem cell), and this increasingly allows for the conduct of cell-based assays using human-derived native cells. In particular, iPS Cardiotoxicity, iPS Neurotoxicity, and iPS Hematotoxicity assessments have been increasingly performed as safety evaluation of compounds. FDSS-μCELL v2 performs high-throughput toxicity screening.
Specifications
| Dispense (96 tip type) A10118-24 |
10 μL ～ 200 μL |
|---|---|
| Dispense (384 tip type) A10118-26 |
1 μL ～ 30 μL |
| Sensor | Hamamatsu qCMOS sensor dedicated to FDSS |
| Sampling rate | 10 Hz (10 data point per second) Maximum with U8524-11 option 120 Hz (120 data point per second) |
| Sampling interval | 0.1 s minimum With U8524-11 option 0.0083 s minimum |
| Light source | 470 nm excitation and 540 nm emission 530 nm excitation and 593 nm emission |
| Plate positions | One stage for assay plate, two stages for compound plate |
| Adaptable microplate | Clear bottom black 96/384 plates (SBS format height 8 mm to 15 mm) |
| Tip/Plate loading | Manual loading |
| Number of sampling data point | 1 to 50 000 sampling |
| Power supply specification | Input power supply: AC 100 V to AC 240 V, Frequency: 50 Hz/60 Hz |
| Power consumption when AC 100 V to AC 120 V (Data Analysis unit and FDSS-μCELL main unit with heater) |
Approx. 1300 VA (Data analysis unit: approx. 500 VA, dispenser main unit: approx. 300 VA, heater unit, approx. 500 VA) |
| Ambient operating temperature | ＋15 ℃ ～ ＋30 ℃ |
| Dimension/weight（Main unit ） *Data analysis unit is not included |
550 mm (W) × 675 mm (D) × 1600 mm (H) / approx. 200 kg |
Dimensions
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