Recently, studies of iPS cells (induced pluripotent stem cells) have made a huge impact in the drug discovery field. Currently, human iPS cell (hiPSC) derived various specific types of cells such as cardiomyocytes and neural cells are now widely available commercially, and the screening of chemical compounds for drug discovery using these hiPSC-derived cells is possible. Screening using hiPSC-derived cells is expected to provide more effective and easy way to evaluate the pharmacological and toxic effects of test compounds in cell-based assays.
HAMAMATSU FDSS series are capable for the measurement and analysis of calcium transient in hiPSC-derived cardiomyocytes.
Both FDSS7000EX and FDSS/μCELL can have the “Heater”, “High Speed Recording” and “Waveform Analysis” option, needed for iPSC cardiomyocyte calcium transient. “Heater” function becomes more and more important when handling iPSC, for its cell status and condition during the assay, especially affects the beating rate of cardiomyocyte. Another key technology and function to monitor the calcium transient is the “High Speed Recording” ability. Maximum around 100Hz sampling rate, shortest around 10ms interval, reading is possible with our high sensitive high speed EM-CCD camera. We also have an option of “Waveform analysis” software, to analyze the number of peak, beating rate, amplitude, rising slope, falling slope PWD from the measured calcium transient data.
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