G-protein-coupled receptors (GPCRs) research and screening are areas that benefit the most from the FDSS kinetic plate imager’s high throughput feature. The FDSS can be equipped with 96- and 384-manifold pipettors to add ligands / compounds into all wells simultaneously, and it images the whole plate so all wells are read at the same time, resulting in no time lag among wells while the reactions of cells occur. GPCRs play a major role in cell signaling, and many GPCR-targeted medical drugs have been developed. Applications (and resources) include:
|Le Poul E, Hisada S, Mizuguchi Y, Dupriez VJ, Burgeon E, Detheux M.||Adaptation of aequorin functional assay to high throughput screening||J Biomol Screen. 2002 Feb;7(1):57-65.|
|Inbe H, Watanabe S, Miyawaki M, Tanabe E, Encinas JA.||Identification and characterization of a cell-surface receptor, P2Y15, for AMP and adenosine||J Biol Chem. 2004 May 7;279(19):19790-9. Epub 2004 Mar 4.|
|McNamara CR, Mandel-Brehm J, Bautista DM, Siemens J, Deranian KL, Zhao M, Hayward NJ, Chong JA, Julius D, Moran MM, Fanger CM.||TRPA1 mediates formalin-induced pain||http://www.ncbi.nlm.nih.gov/pubmed/17686976|
|Cassutt KJ, Orsini MJ, Abousleiman M, Colone D, Tang W.||Identifying nonselective hits from a homogeneous calcium assay screen||J Biomol Screen. 2007 Mar;12(2):285-7. Epub 2007 Feb 8.|
|Wise H, Abel PW, Cawkill D.||Use of reduced temperature cell pausing to enhance flexibility of cell-based assays||J Biomol Screen. 2009 Jul;14(6):716-22. Epub 2009 May 21.|
|Lin Tian, S. Andrews Hires, Tianyi Mao, Daniel Huber, M. Eugenia Chiappe, Sreekanth H. Chalasani, Leopoldo Petreanu, Jasper Akerboom, Sean A. McKinney, Eric R. Schreiter, Cornelia I. Bargmann, Vivek Jayaraman, Karel Svoboda and Loren L. Looger||Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators||Nature Methods 6, 875 - 881 (2009), online: 8 November 2009 | doi:10.1038/nmeth.1398|
|Pantel J, Williams SY, Mi D, Sebag J, Corbin JD, Weaver CD, Cone RD.||Development of a high throughput screen for allosteric modulators of melanocortin-4 receptor signaling using a real time cAMP assay||Eur J Pharmacol. 2011 Jun 11;660(1):139-47. Epub 2011 Feb 4.|
|Jasper Akerboom, Tsai-Wen Chen, Trevor J. Wardill, Lin Tian, Jonathan S. Marvin, Sevinç Mutlu, Nicole Carreras Calderón, Federico Esposti, Bart G. Borghuis, Xiaonan Richard Sun, Andrew Gordus, Michael B. Orger, Ruben Portugues, Florian Engert, John J. Macklin, Alessandro Filosa, Aman Aggarwal, Rex A. Kerr, Ryousuke Takagi, Sebastian Kracun, Eiji Shigetomi, Baljit S. Khakh, Herwig Baier, Leon Lagnado, Samuel S.-H. Wang, Cornelia I. Bargmann, Bruce E. Kimmel, Vivek Jayaraman, Karel Svoboda, Douglas S. Kim, Eric R. Schreiter and Loren L. Looger||Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging||This article is freely available online through the J Neurosci Open Choice option. August 1, 2012|
|Olaposi I. Omotuyi, Jun Nagai & Hiroshi Ueda||Lys39-Lysophosphatidate Carbonyl Oxygen Interaction Locks LPA1 N-terminal Cap to the Orthosteric Site and partners Arg124 During Receptor Activation||Scientific Reports 5, Article number: 13343 (2015)doi:10.1038/srep13343|
|App. Note N°.||Title|
|FDSS Application Note N°. 14||FDSS Application Note No.14 Evaluation of a new intracellular calcium fluorescent reagent using the FDSS6000 [0.4MB/PDF]|
|FDSS Application Note N°. 20||FDSS Application Note No.20 New Tools for Drug Discovery: Multiplexing the monitoring of Ca2+ and cAMP signaling in primary cells using the FDSS/μCell. [0.4MB/PDF]|
|FDSS Application Note N°. 23||FDSS Application Note No.23 BRET1-assay using the FDSS/µCELL imaging plate reader [0.13MB/PDF]|
|2015||In vitro predictive toxicity of GPCR modulators in human iPS-derived Cor.4U cardiomyocytes by Ca2+ transients detection|
|2015||An integrated approach to study the cellular mechanisms of GPCR drug actions using calcium and label-free based platforms|
|2014||The challenge of interpreting drug potency under non-equilibrium conditions using Ca2+ assays|
|2014||FDSS/µCell implementation for medium-throughput calcium assays|
|2014||BRET1-assay using thr FDSS/µCell imaging plate reader: monitoring agonist-induced β-arrestin recruitment to a G protein-coupled receptor (GPCR)|
|2014||An In-vitro Model of Acute Epilepsy Suitable for MTS|
|2014||Importance of calcium assay parameters in drug discovery|
|2014||Integration of FDSS7000 into a modular robotic system for Open Innovation drug discovery|
It looks like you’re in the . If this is not your location, please select the correct region and country below.
You're headed to Hamamatsu Photonics website for U.K. (English). If you want to view an other country's site, the optimized information will be provided by selecting options below.
For modern websites to work according to visitor’s expectations, they need to collect certain basic information about visitors. To do this, a site will create small text files which are placed on visitor’s devices (computer or mobile) - these files are known as cookies when you access a website. Cookies are used in order to make websites function and work efficiently. Cookies are uniquely assigned to each visitor and can only be read by a web server in the domain that issued the cookie to the visitor. Cookies cannot be used to run programs or deliver viruses to a visitor’s device.
Cookies do various jobs which make the visitor’s experience of the internet much smoother and more interactive. For instance, cookies are used to remember the visitor’s preferences on sites they visit often, to remember language preference and to help navigate between pages more efficiently. Much, though not all, of the data collected is anonymous, though some of it is designed to detect browsing patterns and approximate geographical location to improve the visitor experience.
Certain type of cookies may require the data subject’s consent before storing them on the computer.
This website uses two types of cookies:
There are two ways to manage cookie preferences.
If you wish to restrict or block web browser cookies which are set on your device then you can do this through your browser settings; the Help function within your browser should tell you how. Alternatively, you may wish to visit www.aboutcookies.org, which contains comprehensive information on how to do this on a wide variety of desktop browsers.
Occasionally, we may use internet tags (also known as action tags, single-pixel GIFs, clear GIFs, invisible GIFs and 1-by-1 GIFs) at this site and may deploy these tags/cookies through a third-party advertising partner or a web analytical service partner which may be located and store the respective information (including your IP-address) in a foreign country. These tags/cookies are placed on both online advertisements that bring users to this site and on different pages of this site. We use this technology to measure the visitors' responses to our sites and the effectiveness of our advertising campaigns (including how many times a page is opened and which information is consulted) as well as to evaluate your use of this website. The third-party partner or the web analytical service partner may be able to collect data about visitors to our and other sites because of these internet tags/cookies, may compose reports regarding the website’s activity for us and may provide further services which are related to the use of the website and the internet. They may provide such information to other parties if there is a legal requirement that they do so, or if they hire the other parties to process information on their behalf.
If you would like more information about web tags and cookies associated with on-line advertising or to opt-out of third-party collection of this information, please visit the Network Advertising Initiative website http://www.networkadvertising.org.
We use third-party cookies (such as Google Analytics) to track visitors on our website, to get reports about how visitors use the website and to inform, optimize and serve ads based on someone's past visits to our website.
You may opt-out of Google Analytics cookies by the websites provided by Google:
We inform you that in such case you will not be able to wholly use all functions of our website.