Professor Alexander Scheeline, PhD, University of Illinois Urbana-Champaign
January 26, 2016
This presentation discusses the trade-offs to consider when choosing (or designing) a spectrometer that will best suit your application. Professor Scheeline begins with an overview of the components that go into an absorbance spectrometer and common arrangements for the instrument. Next, he explores absorption spectrometry by examining error propagation concepts and expressing them through Beer’s Law. This is followed by details on the many sources of uncertainty that affect measurements, such as errors in concentration, errors in absorbance, digitization noise, light source flicker, shot noise, and dark current. Combinations of some of these noise sources are also discussed. Finally, three measurement examples are presented for examination, along with protein assay sub-examples.
Alexander Scheeline, PhD, is Professor Emeritus, Department of Chemistry, University of Illinois Urbana-Champaign. Dr. Scheeline is also President of SpectroClick Inc. and an honorary member of the Society for Applied Spectroscopy.
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