FDSS-GX kinetic Plate Imager C15711-02

FDSS-GX is the highest-performance kinetic plate imager in the FDSS series, optimized for 96-, 384-, and 1536-well for reliable and stable high throughput screening (HTS), enabling whole microplate imaging and simultaneous injections to all wells. This system achieves high sensitivity imaging with qCMOS sensor, and highly accurate and repeatable micro-dispensing by adopting a 1536 ch dispensing unit capable of dispensing variable volumes. By combining optional parts, a robot-free semi-automation system can be built according to the user's purpose.

FDSS is a registered trademark of Hamamatsu Photonics K.K. (China, France, Germany, Italy, Japan, UK, USA).

Catalog 4.7 MB/PDF

Features
Applications
Specifications
Dimensional outlines
Related information

Features

1. 1536 ch manifold pipettor head with independent metal piston cylinders

Dispensing accuracy and repeatability are important factors in performing assays.

The FDSS-GX achieves highly accurate and repeatable micro-dispensing by adopting a 1536 ch dispensing unit capable of dispensing variable volumes. This dispensing unit has independent metal piston cylinders and dispensing tips dedicated to the FDSS-GX.

Dispensing units in 96 ch and 384 ch are also available.

Dispensing unit (1536 tip type) A15623-28

1536 black tip (10 racks) for FDSS-GX A8687-82

● Dispensing performance

The figure below shows the fluorescence intensity change in time and plate image when B-beads were dispensed into a 1536 microplate (5 µL/well), showing a CV of 1.89 % after dispensing.

The 1536 ch dispensing unit with independent metal piston cylinders and dispensing tips dedicated to FDSS provide the best dispensing performance.

*This is the actual measurement value when dispensing B-beads (5 µL/well) with overhauled or calibrated 1536 ch dispensing unit and dispensing tips dedicated to FDSS-GX.

2. High sensitivity and high-resolution imaging with qCMOS^® sensor

The high sensitivity qCMOS sensor comes as a standard in the FDSS-GX optical system.

In fluorescence and luminescence measurements, high quantitative performance has been achieved for low-light imaging, and the assay window has been expanded due to the sensor's wide dynamic range, enabling measurement with a wide linearity range.

In addition, the qCMOS sensor makes it possible to image the whole plate due to the large sensor size, and enables high-resolution measurement.

● Detection sensitivity in fluorescence

In fluorescence measurements, the FDSS-GX was able to detect 0.03 nM Fluorescein* (exposure time: 50 ms).

The graph below shows the fluorescence count and S/N ratio for titrated concentrations of Fluorescein, and the image below shows the 384 microplate.

* This is the actual measurement value.

Fluorescein concentration vs. RFU

S/N ratio

Microplate image

(384 microplate)

● Detections sensitivity in luminescence

In luminescence measurements, the FDSS-GX was able to detect 3.9 fg Luciferase*(exposure time: 5 seconds) according to the calculations.

The graph below shows the luminescence count for titrated concentrations of Luciferase, and the image below shows the 384 microplate.

* This is the theoretical value calculated from the measurement data below.

Luciferase (pg/well) vs. RLU

Microplate image
(384 microplate)

● Comparison of whole plate imaging

The qCMOS sensor makes it possible to image the whole plate due to the large sensor size, and enables high-resolution measurement .

(1536 plate, Sample: B-Beads)

3. High-precision epifluorescence optical system with high power and long life Xe lamp

The FDSS-GX includes high-precision epifluorescence optical system with high power and long life Xe lamp developed in-house. The combination of Xe lamp and the dedicated dichroic mirrors/optical filters enables measurement using various fluorescent dyes in the UV to IR range.

For details, please refer to the table below.

Epifluorescence optical system

User application
	Excitation filter 1	Excitation filter 2	Shortpass filter	Dichroic mirror	Emission filter 1	Emission filter 2
Fura-2 (Ca²⁺)	340 nm*	387 nm*	SPF 495	UV*	520 nm ～ 560 nm	-
SBFI (Na⁺)	340 nm*	387 nm*	SPF 495	UV*	520 nm ～ 560 nm	-
PBFI (K⁺)	340 nm*	387 nm*	SPF 495	UV*	520 nm ～ 560 nm	-
MQAE, diH-MEQ (CI^-)	387 nm*	-	SPF 495	UV*	440 nm ～ 470 nm*	-
DAPI (DNA)	387 nm*	-	SPF 495	UV*	440 nm ～ 470 nm*	-
Hoechst33258	387 nm*	-	SPF 495	UV*	440 nm ～ 470 nm*	-
VSP-1	387 nm*	-	SPF 400*	For VSP*	466 nm*	560 nm*
CFP/YFP	438 nm*	-	SPF 450*	For C/Y*	483 nm*	542 nm*
HYPER	425 nm*	483 nm*	SPF 495	For HYPER*	520 nm ～ 560 nm	-
Fluo-8 (Ca²⁺)	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
Cal-520 (Ca²⁺)	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
Sodium Green (Na⁺)	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
FluxOR (K⁺)	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
BCECF (pH)	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
FluoVolt	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
GFP	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
FITC	472 nm	-	SPF 495	B	520 nm ～ 560 nm	-
YFP	472 nm	-	SPF 495	For YFP*	520 nm ～ 560 nm	-
JC-1	531 nm	-	SPF 550	For JC-1*	593 nm	-
CoroNa Red (Na⁺)	531 nm	-	SPF 550	For FMP	593 nm	-
FMP	531 nm	-	SPF 550	For FMP	593 nm	-
Cal-590 (Ca²⁺)	531 nm	-	SPF 550	For FMP	593 nm	-
Rhodamine	531 nm	-	SPF 550	For FMP	593 nm	-
Cal-630 (Ca²⁺)	605 nm*	-	SPF 630*	For RED*	676 nm*	-
Nano-BRET	-	-	-	-	461 nm*	647 nm*

*: Option

The wavelength value in the table above may not correspond to the typical excitation/emission wavelength for each application.
Depending on the application, we offer filter sets suitable for the FDSS-GX optical system. Please contact your Hamamatsu representative for further information.

4. Plate stacker unit for semi-automation

The FDSS-GX can automatically transfer assay plates and compound plates by two transfer lines, front and rear. In addition, plate stacker unit with large-capacity of cassette for up to 20 microplates is available for robot-free semi-automation.

FDSS-GX inner layout

Plate stacker unit

Applications

Fluorescence applications

● Ca²⁺ assays

Fluo-8, Calbryte, -520, -590, -630 AM, Fura-2

Evaluation of ATP dose response using Fluo-8 AM-stained CHO cells

● Membrane potential assays

FluoVolt, SQMP, FMP

● Other ion channel assays

K⁺: FluxOR^＊, Na⁺: ANG2, Sodium Green, Cl^-: YFP, diH-MEQ

*Potassium ion channel screening using Tl⁺ is available.

K⁺ assay in CHO cells using FluxOR

● FRET assays

CFP/YFP, voltage sensor probe, fluorescence probe, fluorescence protein

Evaluation of Nav 1.5-CHO cells using FRET-type voltage sensitive dye (VSP)

● iPSC-derived cells

Ca²⁺ transient and membrane potential measurements using iPS cell-derived cardiomyocytes

Luminescence applications

● Ca²⁺ assays

Aequorin

Intracellular Ca²⁺ assay by luminescence using an aequorin-expressing cell line

Ca²⁺ assay using aequorin-expressing cells

● BRET assays

BRET1, BRET2, NanoBRET

BRET assay using CHO cells

principle of β-arrestin BRET¹- assay

Rluc - D2s receptors and eYFP – β-arrestin 2 are expressed in CHO cells, and Rluc and eYFP BRET occurring in cells when Ligand is added are detected.

● cAMP assays

GloSensor

cAMP evaluation using GloSensor

Analysis of time course of cAMP using HUVEC expressing GloSensor (Promega)

● Cell number evaluation using CytoTox-Glo cytotoxicity Assay

Cell number evaluation using CytoTox-Glo cytotoxicity Assay

Specifications

Dispensing units	1536 tip type A15623-28	1 µL to 5 µL
	384 tip type A10118-26	1 µL to 30 µL
	96 tip type A10118-24	10 µL to 200 µL
Fluorescence and luminescence detector		Hamamatsu qCMOS sensor dedicated to FDSS
Number of sampling data points		1 to 50 000 sampling
Sampling rate		10 Hz (10 data points per second) minimum 120 Hz (120 data points per second) maximum*
Sampling interval		0.1 s minimum 0.0083 s minimum*
Excitation light source		Xe lamp Optical filters can be selected
Plate shaking function		Adjustable in 256 steps Revolutions per minute: 0~3000 rpm
Heater unit	Temperature control part	FDSS-GX Main unit chamber (whole upper half)
Heater unit	Configurable temperature	+40 ºC maximum
Tip loading		Use Automatic tip loader A15623-07
Number of plate loading lines		Front loader × 1　Rear loader × 1
Number of plate positions	Assay plate	Front loader × 1
Number of plate positions	Compound plate	Rear loader × 1, Compound plate stage (manual exchange) × 3
Number of tip washer positions		3 units maximum (Total with/without high power ultrasonic function, deep reservoir)
Number of dispensing unit positions		2 units maximum (same tip type only)
Compatible dispensing tip		1536 / 384 / 96 black tips dedicated to FDSS, A8687 series Disposable type
Compatible microplate		Clear bottom black 1536 / 384 / 96 plates SBS format height 10 mm minimum
Power supply specifications		Single phase AC 100 V ~ 240 V, 50 Hz / 60 Hz
Power consumption		C15711-02: approx. 3115 VA
		C7903-13: approx. 660 W
		Required supply line: 15 A × 3 lines
Ambient operating temperature		+15 ºC to 30 ºC
Dimensions/Weight	Main unit	1437 (W) × 850 (D) × 1467 (H) mm / approx. 320 kg
	Data analysis unit	570 (W) × 880 (D) × 943 (H) mm / approx. 82 kg
	Plate stacker set	437 (W) × 580 (D) × 1340 (H) mm / approx. 36 kg
	Washing rack	616 (W) × 924 (D) × 1038 (H) mm / approx. 19 kg
	Circulating water cooler	222 (W) × 386 (D) × 649 (H) mm / approx. 26 kg
	Automatic tip loader	233 (W) × 289 (D) × 447 (H) mm / approx. 21 kg