Features | Light-Sheet Microplate Cytometer

Three key features

CYTOQUBE has the following three features

  • High-throughput
  • Simple scanning and analysis settings
  • Enhanced realism and accuracy in 3D fluorescent imaging

These features contribute to the efficiency of drug screening, toxicity/safety evaluation, and translational research using cells, spheroids, organoids, and bioprinting samples.

High-throughput

Rapid scanning speed

Acquire whole-well and whole-plate fluorescence images within a few minutes per color, regardless of plate format (1536, 384, 96 wells) or sample depth.

Parallel processing for scanning and analysis

The parallel processing of fluorescence image construction and image analysis has significantly shortened the time from scan start to data analysis result output.

Simple scanning and analysis settings

Easy setting

The settings have been simplified to make it easy to use. Unnecessary settings such as 'focus adjustment' or 'exposure time adjustment' are not included. The optimal sensitivity can be set automatically with the Navigation Scan function. Additionally, you can save the entire process from scanning to result display as a protocol file, making it easy to reproduce scanning and analysis conditions.

Intuitive user interface

Settings required for image analysis can be set intuitively, as necessary settings are summarized for each application.

Complete scanning & analysis software in one package

A single software can be used to check 2D/3D fluorescence images of each well, calculate EC50/IC50, create various graphs, identify hit wells, and generate simple reports.

3D fluorescent image display

Scatter plots & gate settings

Hit-well identification

Enhanced realism and accuracy in 3D fluorescent imaging

Real-time background separation

Background fluorescence, derived from autofluorescence of the culture medium or fluorescent dyes in the solution, and fluorescence from the sample are separated from the acquired XZ tomographic fluorescence images, in real-time, effectively removing only the background fluorescence in three dimensions.

 

A549 cells cultured with DMEM medium, containing serum and fluorescent dye, were measured both in a fluorescence microscope and with CYTOQUBE to compare the images.

Fluorescent dyes: Annexin-V-Alexa Fluor 488

Real-time background separation OFF

Real-time background separation ON

Seamless 3D fluorescent images

In a typical confocal microscope, multiple 2D XY images at different depths are acquired to construct a 3D fluorescence image. To obtain more accurate 3D information, a larger number of image acquisitions are required.

CYTOQUBE consistently provides seamless 3D fluorescence images using Zyncscan technology, ensuring precise 3D information without the need for extensive image acquisition.

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