High-throughput screening (HTS) and high-content screening (HCS) are techniques used to study biological systems simultaneously, and are commonly used in cell biology and pharmaceutical drug discovery. For instance, when studying the effects of a drug on a specific bacterium, samples of the bacterium are placed in small compartments called wells in a biological test. Different amounts of the drug are then added to the wells, and all the wells are examined at the same time using various methods like fluorescence microscopy, Raman scattering, and confocal microscopy. These tests are automated, allowing thousands to millions of experiments to be conducted to test the effectiveness of different compounds in treating a disease and to determine the efficacy of promising drugs.
HTS/HCS systems involve several complex instruments working together. These include a fluid handler that changes the substances used in the test, a robot that moves plates with 96 or 384 wells in and out of the testing area, and an imaging device to capture data. Once the data is collected, sophisticated software is used to analyze it and identify compounds that show potential for further evaluation. These systems form the foundation of modern drug discovery, efficacy testing, and validation.
High speed, kinetic plate imager for fluorescence / luminescence assays in GPCR / ion channel drug discovery field.
The low noise and high resolution properties of the sCMOS cameras satisfy the sensitivity and quantitative requirements for high content screening in a short exposure time.
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