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High-throughput screening/
High-content screening
High-throughput screening (HTS) and high-content screening (HCS) are techniques used to study biological systems simultaneously, and are commonly used in cell biology and pharmaceutical drug discovery. For instance, when studying the effects of a drug on a specific bacterium, samples of the bacterium are placed in small compartments called wells in a biological test. Different amounts of the drug are then added to the wells, and all the wells are examined at the same time using various methods like fluorescence microscopy, Raman scattering, and confocal microscopy. These tests are automated, allowing thousands to millions of experiments to be conducted to test the effectiveness of different compounds in treating a disease and to determine the efficacy of promising drugs.
HTS/HCS systems involve several complex instruments working together. These include a fluid handler that changes the substances used in the test, a robot that moves plates with 96 or 384 wells in and out of the testing area, and an imaging device to capture data. Once the data is collected, sophisticated software is used to analyze it and identify compounds that show potential for further evaluation. These systems form the foundation of modern drug discovery, efficacy testing, and validation.
Recommended products
Kinetic plate imager
High speed, kinetic plate imagers for fluorescence / luminescence assays in GPCR / ion channel drug discovery field.
Introducing the highest-performance kinetic plate imager in the FDSS series, the C15711-02, optimized for 1536 / 384 / 96 wells for sensitive, reliable, and precise high throughput screening (HTS). It enables whole microplate imaging and simultaneous injection into all wells featuring:
- a high sensitivity two-dimensional qCMOS sensor for fluorescence and luminescence analysis
- 1536 ch manifold pipettor head with independent metal piston cylinders
- dedicated optics to measure all wells uniformly
- semi-automation with stackers - automatically transfer assay plates and compound plates by two transfer lines, front and rear.
A compact, affordable, easy to use kinetic plate imager optimized for 384 / 96 wells for sensitive, reliable, and precise high throughput screening (HTS). It enables whole microplate imaging and simultaneous injection into all wells featuring:
- a high sensitivity two-dimensional EM-CCD sensor for fluorescence and luminescence analysis
- 384/96 ch manifold pipettor head with independent metal piston cylinders
- dedicated optics to measure all wells uniformly
- long life and stable LED light source with 2 wavelength measurement options
Scientific CMOS cameras
The low noise and high resolution properties of the sCMOS cameras satisfy the sensitivity and quantitative requirements for high content screening in a short exposure time.
The ORCA-Fusion BT is performance without compromise. This camera hits an imaging sweet-spot regarding resolution, speed, and back-thinned quantitative low noise low-light performance at 60x & 100x. Our patented lighsheet readout modes meet the needs of the most demanding applications.
The Fusion personifies robust quantitative versatility. It’s optimized for low-light performance and affordability. Imaging at 60-100x? You will be impressed.
The ORCA-Flash4.0 V3 delivers reliable performance. For standard brightness specimens it will do very well, but for super low-light applications the lower noise of the Fusions, Quest2, and Fire may be more appropriate. If you are looking for quality & performance, on a budget, consider a Flash4.0 V3; it’s been popular in labs for over a decade of research.
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