ORCA-Flash4.0 V3 Digital CMOS camera
Building on our extensive experience with high performance scientific cameras and advanced imaging applications, Hamamatsu introduces the new ORCA-Flash4.0 V3. This one camera expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification and speed. With on-board FPGA processing enabling intelligent data reduction, highly refined in-camera, pixel-level calibrations, increased USB3.0 frame rates, purposeful and innovative triggering capabilities, patented lightsheet read out modes and individual camera noise characterization the ORCA-Flash4.0 V3 is the precision instrument for imaging.
“Lightsheet Readout Mode” is a unique and patented feature of Hamamatsu sCMOS cameras which can improve signal to noise ratios in Lightsheet microscopy.
For more information about the principle and features of Lightsheet Readout Mode, please see details from below.
With the introduction of the ORCA-Flash4.0, users are now able to stream 4 megapixel images to their computers 100 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0.
|Quantum efficiency||82 % (peak QE: @560 nm)|
|Effective no. of pixels||2048 (H)×2048 (V)|
|Cell size||6.5 μm×6.5 μm|
|Effective area||13.312 mm×13.312 mm|
|Full well capacity||30 000 electrons (typ.) *|
|Readout speed||100 frames/s (Full resolution, standard scan, Camera Link) *
40 frames/s (Full resolution, standard scan, USB 3.0, 16 bit) *
53 frames/s (Full resolution, standard scan, USB 3.0, 12 bit) *
80 frames/s (Full resolution, standard scan, USB 3.0, 8 bit) *
|Readout noise||Standard scan (at 100 frames/s, typ.):1.6 electrons rms (1.0 electrons median)
Slow scan (at 30 frames/s, typ.): 1.4 electrons rms (0.8 electrons median)
|Cooling method||Peltier cooling|
|Cooling temperature||Forced air (Ambient at +20 ℃): -10 ℃
Water (+20 ℃): -10 ℃
Water (+15 ℃): -30 ℃
|Dark current||0.06 electrons/pixel/s (Air Cooled to -10° C) (typ.)
0.06 electrons/pixel/s (Water Cooled to -10° C) (typ.)
0.006 electrons/pixel/s (Water Cooled to -30° C) (typ.)
|Dynamic range||37 000:1 (typ.)|
|Interface||Camera Link / USB 3.0|
|A/D converter||16 bit / 12 bit / 8 bit|
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