ORCA-Flash4.0 LT3 Digital CMOS camera


The ORCA-Flash4.0 LT3 is a new scientific CMOS camera for fluorescence imaging, which has been improved from the ORCA-Flash 4.0 released in 2011.

It is equipped with a high level of performance required for fluorescence imaging such as life science applications, and can be used not only for basic research applications but also for integration into various types of equipment.


  • Low readout noise (Standard scan): 1.5 electrons rms, 0.9 electrons median
  • High speed readout (Rapid rolling, Full resolution): 40 frames/s
  • Large field of view: 13.312 mm (H)×13.312 mm (V)
  • High resolution: 2048 (H)×2048 (V)
  • High quantum efficiency: 82 % (Peak QE)


  • Time-lapse imaging
  • Multidimensional imaging
  • Ca2+ imaging
  • DNA chip reading

High S/N equivalent or better than BSI camera

The graph below compares the readout noise distribution with the Gen II BSI camera* and shows that the ORCA-Flash4.0 LT3 has more pixels with smaller readout noise.

With a low readout noise of 1.5 electrons rms, the ORCA-Flash4.0 LT3 delivers high S/N comparable to or better than a BSI camera, especially in the low light range.

This performance can be demonstrated in high quality multidimensional imaging, as typified by long time-lapse imaging with low excitation light with low phototoxicity and photobleaching.

* BSI camera: Back-side illuminated camera

High speed readout

The ORCA-Flash4.0 LT3 is capable of 40 frames/second readout, which is even faster than the conventional ORCA-Flash4.0 LT+ (30 frames/second). This is useful for applications which requires real time measurement with high temporal resolution.

Large field of view

The wide field of view of 13.312 mm (H) × 13.312 mm (V) allows more information to be obtained in a single shot. 

This contributes to higher throughput in experiments such as DNA chip reading.

PC recommendations

With the introduction of the ORCA-Flash4.0 LT3, users are now able to stream 4 megapixel images to their computers 40 frames per second. The computer recommendations for this high data rate can be met by using the guidelines listed this PC Recommendations for ORCA-Flash4.0 LT3.


Our software provides the interface to access all of our carefully engineered camera features, from simply setting exposure to orchestrating complex triggering for multidimensional experiments.


Product number C11440-42U40
Imaging device Scientific CMOS image sensor
Effective number of pixels 2048 (H) × 2048 (V)
Pixel size 6.5 μm (H) × 6.5 μm (V)
Effective area 13.312 mm (H) × 13.312 mm (V)
Full well capacity (typ.) 30 000 electrons
Dynamic range (typ.) 33 000: 1 *1 20 000: 1 *2
Cooling method Forced-air cooled, peltier cooling
Cooling temperature +10 ℃ (Forced-air cooled, FAN speed: Fast ambient temperature: +10 °C to +30 °C)
Frame rate at full resolution Rapid rolling : 40 frames/s
Standard scan : 30 frames/s
Readout noise (typ.) Rapid rolling : 1.9 electrons (rms) , 1.3 electrons (median)
Standard scan : 1.5 electrons rms / 0.9 electrons median
Dark current (typ.) 0.6 electrons/pixel/s (typ.) (Cooling temperature: +10 °C)
Digital output 16 bit
Exposure time Internal trigger mode *3 (Rapid rolling) : 1 ms to 10 s
Internal trigger mode *3 (Standard scan) : 3 ms to 10 s
Internal trigger mode with sub-array readout (Rapid rolling) : 40 μs to 10 s
Internal trigger mode with sub-array readout (Standard scan) : 129.99 μs to 10 s
External trigger input mode Edge trigger, Global reset edge trigger, Level trigger, Global reset level trigger, Synchronous readout trigger, Start trigger
Trigger delay function 0 s to 10 s in 1 μs steps
Trigger input connector SMA
Trigger output 3 programmable timing outputs, Trigger ready output, Global exposure timing output, Low output, High output
Trigger output connector SMA
Binning 2×2, 4×4
Sub-array Yes
Interface USB 3.1 Gen 1
Lens mount C-mount
Power supply AC 100 V to AC 240 V, 50 Hz / 60 Hz
Power consumption Approx. 75 VA
Ambient operating temperature 0 °C to +40 °C (FAN speed: Fast)
Ambient operating humidity 30 % to 80 % (With no condensation)
Ambient storage temperature −10 °C to +50 °C
Ambient storage humidity 90 % Max. (With no condensation)

*1  Calculated from the ratio of the full well capacity and the readout noise (median) in standard scan

*2  Calculated from the ratio of the full well capacity and the readout noise (rms) in standard scan

*3  Minimum exposure time in internal trigger mode varies depending on sub-array size and position.

Spectral response


Instruction manual

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It introduces technical information, simulation tools, and examples of actual applications to help you fully understand the performance of the camera and select the best one for your application.

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