Achieving more with less.

Archive of CYTO commercial tutorial

Commercial Tutorials are educational sessions held as part of the CYTO conference program, where companies and industry experts introduce new technologies, measurement approaches, and application possibilities in flow cytometry.

Unlike a general product introduction, these sessions provide an opportunity to explore the technical background of emerging solutions, examine experimental data, and discuss how new technologies may address challenges in current flow cytometry workflows.

CYTO2024

Introducing a New Hybrid Sensor for Flow Cytometry

This tutorial introduces HYPEREON, a hybrid photosensor developed for flow cytometry. It explains how HYPEREON combines electron-tube and solid-state technologies and explores its potential through data analysis, simulations, and application examples.

CYTO2025

Exploring the Value of HYPEREON in Flow Cytometry

Building on the CYTO 2024 tutorial, this presentation explores the value of HYPEREON in flow cytometry. Experimental results and application examples illustrate its potential for spectral analysis, instrument standardization, and nanoscale particle measurement.

The information in this archived presentation reflects the status at the time and may differ from the latest specifications or development status.

1. Measurement of four mixed samples

Measurement of four mixed samples with one laser and one gain operation

4 mixed samples

Sample 1 2 3 4
APC 30 ng 0 ng 30 ng 15 ng
Alexa Fluor 647 0 ng 30 ng 15 ng 30 ng

 

Compensation Bead;  BD™ Anti-Rat Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set [Cat. No. 560499]

Fluorescence Antibody Reagent; APC anti-mouse CD62L Antibody [Cat. No. 161217]

Alexa Fluor 647® anti-mouse CD197 (CCR7) Antibody [Cat. No. 120111]

Buffer; BD PharmingenTM Stain Buffer(FBS) [Cat. No. 554656]

Separation accuracy varies depending on the channel selected

Hypereon

Acceptable

APC:657

AF647:670

Optimal

APC:657

AF647:684

Suboptimal

APC:670

AF647:684

Optimal

APC:657

AF647:697

PMT

Unacceptable

APC:670

AF647:695

Unacceptable

APC:644

AF647:695

Unacceptable

APC:670

AF647:718

2. Measurement of 13 mixed samples

Measurement of 13 mixed samples with one laser and one gain operation

APC:657, AF647:684

13 mixed samples

Sample 1 2 3 4 5 6 7 8 9 10 11 12 13
Sample ratio APC Single stain Alexa Fluor 647 Single stain APC+Alexa Fluor 647
APC 160 ng 40 ng 3.2 ng 0.8 ng 0 ng 0 ng 0 ng 0 ng 160 ng 40 ng 20 ng 3.2 ng 1.2 ng
Alexa Fluor 647 0 ng 0 ng 0 ng 0 ng 160 ng 40 ng 3.2 ng 0.8 ng 160 ng 20 ng 40 ng 3.2 ng 1.2 ng

 

Compensation Bead;  BD™ Anti-Rat Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set [Cat. No. 560499]

Fluorescence Antibody Reagent; APC anti-mouse CD62L Antibody [Cat. No. 161217]

Alexa Fluor 647® anti-mouse CD197 (CCR7) Antibody [Cat. No. 120111]

Buffer; BD PharmingenTM Stain Buffer(FBS) [Cat. No. 554656]

3. Measurement examples for nano particle: EV measurement

Optics for comparison of Hypereon and PMT

Data provision and cooperation

Yuichiro Iwamoto

Postdoctoral Researcher, Ota Lab, The University of Tokyo

Specialized in high-throughput, single-particle analysis by integrating optics, fluidics, and data science.

Hypereon enables “good” detection of <10 photons

Raw data from Hypereon, PMT and ground truth

  1. Detect events and count photons using ground truth
  2. Judge detection by PMT and HYPEREON for each event
  3. Calculate accuracy as mAP (mean Average Precision)
  4. Plot mAP vs. photon count for each detector

Estimation of detectable Particle diameter

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