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Digest movie

Ex1 Measurement of four mixed samples

Measurement of four mixed samples with one laser and one gain operation

4 mixed samples

  • APC Single stain (30 ng)
  • AF647 Singl stain (30 ng)
  • APC+AF647 (30 ng:15 ng)
  • APC+AF647 (15 ng:30 ng)

 

Compensation Bead;  BD™ Anti-Rat Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set [Cat. No. 560499]

Fluorescence Antibody Reagent; APC anti-mouse CD62L Antibody [Cat. No. 161217]

       Alexa Fluor® 647 anti-mouse CD197 (CCR7) Antibody [Cat. No. 120111]

Buffer; BD PharmingenTM Stain Buffer(FBS) [Cat. No. 554656]

Separation accuracy varies depending on the channel selected.

Hypereon

Good

APC:657

AF647:670

Excellent

APC:657

AF647:684

Not Good

APC:670

AF647:684

Excellent

APC:657

AF647:697

PMT

Bad

APC:670

AF647:695

Bad

APC:644

AF647:695

Bad

APC:670

AF647:718

Ex2 Measurement of ten mixed samples

Measurement of ten mixed samples with one laser and one gain operation

10 mixed samples

  • APC Single stain 160ng
  • APC Single stain 40ng
  • APC Single stain 3.2 ng
  • AF647 Single stain 160ng
  • AF647 Single stain 40ng
  • AF647 Single stain 3.2 ng

  • APC + AF647 160ng : 160ng
  • APC + AF647 40ng : 20 ng
  • APC + AF647 20ng : 40ng
  • APC + AF647 3.2ng : 3.2ng

 

Compensation Bead;  BD™ Anti-Rat Ig, κ/Negative Control (BSA) Compensation Plus (7.5 µm) Particles Set [Cat. No. 560499]

Fluorescence Antibody Reagent; APC anti-mouse CD62L Antibody [Cat. No. 161217]

       Alexa Fluor® 647 anti-mouse CD197 (CCR7) Antibody [Cat. No. 120111]

Buffer; BD PharmingenTM Stain Buffer(FBS) [Cat. No. 554656]

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