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CYTOQUBE ライトシートマイクロプレートサイトメータ
C15200-01RGBU
CYTOQUBE ライトシートマイクロプレートサイトメータは、蛍光染色した2次元および3次元の細胞試料を蛍光イメージング・解析するシステムです。1536、384、96 ウエルマイクロプレートに対応し、ホールウエル3次元蛍光画像を高速に取得します。画像取得と同時に、個々の細胞や個々のオルガノイドの蛍光強度および3次元の形態情報を解析し、結果を表示します。
CYTOQUBE、Zyncscanは、浜松ホトニクス株式会社の登録商標です。
3つの特長
CYTOQUBEは以下の3つの特長を持っています。
- 圧倒的なスループット
- シンプルなスキャンと解析
- 正確な3次元蛍光イメージング
創薬スクリーニングや毒性・安全性評価の効率向上、トランスレーショナル研究の推進に貢献します。
比類なきスキャンスピード
1536、384、96ウエルのプレートフォーマットやサンプル厚さによらず、ホールプレート、ホールウエルの蛍光画像を、1色あたり数分の速さで取得します。
スキャンと解析の並列処理
蛍光画像の構築と画像解析を並列処理することで、スキャン開始からデータ解析結果出力までの時間を大幅に短縮しました。
簡単設定
フォーカス調整や露光時間調整など、煩雑なスキャン設定は一切不要です。
Navigation scan機能により、最適な感度を自動設定することができます。
また、スキャンから結果表示までをプロトコルファイルとして保存できるため、スキャン・解析条件を簡単に再現することができます。
シンプルなユーザーインターフェース
アプリケーションごとに、画像解析に必要な設定項目がまとめられているため、直感的に画像解析の設定を行えます。
スキャンから解析まで1つのソフトウエアで完結
スキャン設定から画像解析、データ解析まで、1つのソフトウエアで完結します。データ解析画面では、各ウエルの2次元/3次元蛍光画像の確認から、EC50/IC50の算出、各種グラフ作成、ヒットウエルの同定、簡単なレポート作成などができます。
3次元蛍光画像表示
散布図・ゲート設定
ヒットウエル同定
リアルタイム自動バックグラウンド蛍光除去
培地の自家蛍光や溶液中の蛍光色素などに由来するバックグラウンド蛍光とサンプル由来蛍光を、実際に取得したXZ蛍光断層画像から自動判別し、 3次元的にバックグラウンド蛍光のみを除去します。
血清・蛍光色素を含むDMEM培地中のA549細胞の蛍光画像を、蛍光顕微鏡とCYTOQUBEで取得しました。
蛍光色素：Annexin V-Alexa Fluor 488
バックグラウンド蛍光除去機能オフ
バックグラウンド蛍光除去機能オン
シームレスな蛍光3次元画像
一般的な共焦点顕微鏡では、深さが異なる2次元XY画像を複数枚取得し、3次元蛍光画像を構築します。より正確な3次元情報を得るためには、より多くの取得枚数が必要になります。
一方、CYTOQUBEはZyncscan技術を用いて、連続的に取得したXZ蛍光断層画像から3次元蛍光画像を構築します。これによりスキャン設定に関係なく、シームレスかつ正確な3次元蛍光イメージングを実現します。
ミニアプリケーションノート
仕様
| 製品名 | CYTOQUBE ライトシートマイクロプレートサイトメータ |
|---|---|
| 型名 | C15200-01RGBU |
| スキャン方式 | ライトシート光学系を用いた高速3 次元蛍光測定方式 （ZyncscanⓇ 技術） |
| 励起レーザ | 405 nm、488 nm、561 nm、637 nm から選択（最大4 種類） |
| 蛍光波長干渉フィルタ | 458 nm、531 nm、600 nm、680 nm から選択（最大6 箇所） |
| イメージセンサ | 科学計測用CMOS イメージセンサ |
| 画像解像度 | 2.75 μm（ X）× 2.75 μm（Y）× 6.215 μm（Z） |
| 観察可能最大高さ※1 | 400 μm |
| フォーカス機能 | フォーカスフリー（Zyncscan 技術） |
| 使用マイクロプレート | 1536、384、96 ウエルマイクロプレート （各社HCS 用グレード、SBS 規格） |
| 温度設定範囲 | +30 ℃ ～ +45 ℃（周囲温度：+25 ℃） |
| CO2濃度監視範囲 | 0 ％ ～ 10 ％（CO2 インレット有） |
| バーコードリーダ | 1 次元 （読み取り位置：マイクロプレートの短辺側面両側）※2 |
| 定格 | AC 100 V ～ AC 240 V |
| 消費電力 | 約700 VA |
| 動作周囲温度 | +20 ℃ ～ +30 ℃ |
| 動作周囲湿度 | 30 ％ ～ 80 ％（結露しないこと） |
※1 マイクロプレート底面の平坦性が短辺方向に±50 μm の場合のウエル底面からの高さ
※2 厚さ9.5 mm 未満の1536、384、96 ウエルマイクロプレートの場合、正常に読み取れない場合があります。
※ オートメーションについてはお問い合わせください。
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