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W-VIEW GEMINI-2C イメージスプリッティング光学系
A12801-10
W-VIEW GEMINI-2Cは、入射光画像を2波長に分光し、それぞれの画像を2台のカメラに結像させる顕微鏡用イメージスプリッティング光学系です。
2波長同時イメージングを超解像性能で実現します。
特長
超解像性能
自社開発された専用レンズの採用により、イメージエリアφ12 mm以内は回折限界が維持でき、超解像イメージングに使用可能です※。
※ 像側NA 0.025以下の対物レンズに対する条件でのレンズや関連部品の製造・組立誤差も考慮した場合のシミュレーション結果です。
１ピクセルレベルの色ズレ抑制
倍率色収差や光学歪による2 波長画像の色ズレが１ピクセルレベルに抑えられています※。 ZOOM 補正レンズユニット A12801-10用（オプション）を使用することで、対物レンズの倍率色収差も補正できます。
※ ピクセルサイズは6.5 μm（ ORCAⓇ-Flash4.0使用時）で計算しています。
高ユニフォミティ
98 %の高いユニフォミティにより、画像劣化が最小限に抑えられ、高精度な2波長イメージングが可能です。
最大98 % の高い透過率
W-VIEW GEMINI-2Cは、蛍光イメージングで使用される様々な蛍光タンパク質や試薬の検出波長である450 nm ～ 800 nm の広い波長範囲において高い透過率を実現しています。暗いサンプルの検出や弱い励起光での観察に使用可能です。
市販のダイクロイックミラー、バンドパスフィルタが使用可能
市販のダイクロイックミラー（38 mm × 26 mm、厚さ 3 mmまで）や市販のバンドパスフィルタ、NDフィルタ（φ25.4 mm、厚み6.0 mmまで）が使用できるため、実験に応じた幅広い光学部品の選択が可能です。詳しくは「光学部品の指定寸法」をご参照ください。
光学部品の指定寸法
| 寸法（mm）/公差（mm） | 厚さ（mm） | ||
|---|---|---|---|
| ダイクロイックミラー | 25.1 × 35.5 ～ 26.1 × 38.1 | 0.9 ～ 3.1 ※1 | |
| ビームスプリッタキューブ | 24.8 × 24.8 ～ 25.8 × 25.8 | 24.8 ～ 25.8 | |
| バンドパスフィルタ | φ25.4 | +0/-0.6 | 最大 6.0 ※2 |
| NDフィルタ | |||
※1 結像特性の良いイメージンググレードと呼ばれる反射側の平坦度の高いもの (厚み3 mmのもの)を使用することを推奨します。
※2 バンドフィルタとNDフィルタの厚みを合わせた値です。
容易な位置合わせ
ユーザー目線の設計により、2波長画像の位置合わせが簡単に行えます。
3波長同時計測
W-VIEW GEMINI-2C は、4f光学系で構成されています。1台目のW-VIEW GEMINI-2Cに理想的な平行光が入力された場合、2台目にも理想的な平行光を出力できます。これにより、2台を組み合わせることにより、高精度な3波長同時計測が可能です。
4波長同時計測
W-VIEW GEMINI-2C に2台のW-VIEW GEMINIを組み合わせて4波長同時計測も可能です。
光路
アプリケーション
- 超解像顕微鏡での2波長同時イメージング
- １分子蛍光観察における2波長同時イメージング
- ライトシート顕微鏡における2波長同時イメージング
参考文献
W-VIEW GEMINI-2C
| 著者 | タイトル | 書名 |
|---|---|---|
| Ling Wang, Zachary Lee Johnson, Michael R Wasserman, Jesper Levring, Jue Chen, Shixin Liu | Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses | eLife 2020;9:e56451 |
| Minami Tanaka,Yuki Fujii,Kazumi Hirano,Takumi Higaki,Akira Nagasaki,Ryoki Ishikawa,Takaharu Okajima,Kaoru Katoh | Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin | Genes Cells. 2019; 24: 202– 213. |
| Zhou Yu, Lauren E Surface, Chong Yon Park, Max A Horlbeck, Gregory A Wyant, Monther Abu-Remaileh, Timothy R Peterson, David M Sabatini, Jonathan S Weissman, Erin K O'Shea | Identification of a transporter complex responsible for the cytosolic entry of nitrogen-containing bisphosphonates | eLife 2018;7:e36620 |
| Minami Tanaka,Yuki Fujii,Kazumi Hirano,Takumi Higaki,Akira Nagasaki,Ryoki Ishikawa,Takaharu Okajima,Kaoru Katoh | Heterotrimeric Gq proteins act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias | Nat Commun 13, 487 (2022). |
| Ryo Yoshizawa, Nobuhisa Umeki, Akihiro Yamamoto, Mariko Okada, Masayuki Murata, and Yasushi Sako | p52Shc regulates the sustainability of ERK activation in a RAF-independent manner | Molecular Biology of the Cell 2021 32:19, 1838-1848 |
| Yoshizawa R, Umeki N, Yamamoto A, Murata M, Sako Y. | Biphasic spatiotemporal regulation of GRB2 dynamics by p52SHC for transient RAS activation | Biophys Physicobiol. 2021 Jan 8;18:1-12. |
仕様
| 品名 | W-VIEW GEMINI-2C イメージスプリッティング光学系 |
|---|---|
| 用途 | 顕微鏡蛍光イメージング用 |
| 型名 | A12801-10 |
| 構造 | カメラ２台タイプ カメラ並列設置構造、倒立型顕微鏡向け |
| 入力マウント | Cマウント（メス） |
| 出力マウント | Cマウント（オス） |
| リレー倍率 | 1.0 倍 |
| リレーレンズ焦点距離 | 130 mm |
| 視野 ※1 | φ20 mm φ12 mm （回折限界）※2 |
| バイパスモード※3 | 有 |
| 透過波長範囲 ※4 | 450 nm ～ 800 nm |
| 最大透過率（Typ.）※5 | 98 % |
| ダイクロイックミラー ※6※7 | 市販のダイクロイックミラーが使用可能 |
| ビームスプリッタキューブ ※6※7 | オプション、市販のビームスプリッタキューブが使用可能 |
| バンドパスフィルタ ※6※7 | 市販のバンドパスフィルタが使用可能 |
| NDフィルタ ※6※7 | 市販のNDフィルタが使用可能 |
| 軸上色収差補正機構 | 有 |
| 倍率色収差補正機構 | オプション |
| 鏡像反転 | 無 |
| X-Y位置調整 | 有 |
| 回転調整機構 | 有 |
| ディストーション(Typ.) ※8 | 0.05 % |
| 色ズレ(Typ.) ※8※9 | 6 μm |
| ユニフォミティ(Typ.) ※10 | 98 % |
| 動作周囲温度 | 0 ℃ ～ +40 ℃ |
| 保存周囲温度 | -10 ℃ ～ +50 ℃ |
| 動作・保存周囲湿度 | 70 %以下（ただし結露しないこと） |
※1 リレーレンズや中間変倍レンズと共に使用した場合にケラレなどが発生する場合があります。
※2 像側NA 0.025の対物レンズを使用したシミュレーションにおいての値です。
※3 ダイクロイックミラーなどを光路から外して、顕微鏡からの入力光画像をそのままカメラに結像させるモードです。
※4 バイパスモード時の値です。
※5 バイパスモード時におけるピーク波長での代表値です。
※6 ダイクロイックミラー・ビームスプリッタキューブ・バンドパスフィルタ・NDフィルタは本体に付属されませんので、別途ご購入が必要です。ご使用には、結像特性の良いイメージンググレードと呼ばれる反射側の平坦度の高いもの（厚み3 mmのもの）を使用することを推奨します。ダイクロイックホルダ（空）とフィルタホルダ（空）は、１セット付属されています。セットの追加はA12802-10の型名で購入が可能です。ビームスプリッタキューブをご使用の場合には、ビームスプリッタキューブホルダA12802-16の購入が必要です。
※7 使用可能なサイズは「光学部品の指定寸法」をご参照ください。
※8 対物レンズなどを含まないW-VIEW GEMINI-2C単体での値です。
※9 中央位置を合わせた時の周辺位置のズレ量を示します。
※10 顕微鏡にORCA-Flash4.0を直接取り付けて測定した画像とW-VIEW GEMINI-2Cを介して測定した画像との比の最大値と最小値の差で示します。
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